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首页> 外文期刊>Journal of Economic Entomology >Evaluation of Metarhizium brunneum F52 (Hypocreales: Clavicipitaceae) for Control of Japanese Beetle Larvae in Turfgrass
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Evaluation of Metarhizium brunneum F52 (Hypocreales: Clavicipitaceae) for Control of Japanese Beetle Larvae in Turfgrass

机译：用于防治草皮甲中日本甲虫幼虫的梅花甲F52（Hypocreales：Clavicipitaceae）的评价

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Experimental and commercial preparations of Metarhizium brunneum (Petch) strain F52 were evaluated for control of Japanese beetle, Popillia japonica Newman (Coleoptera: Scarbaeidae), larvae (white grubs) in the laboratory and under field conditions. Experimental preparations consisted of granule and liquid formulations made using in vitro produced microsclerotia, which are intended to produce infective conidial spores after application. These formulations were compared against commercial insecticides (imidacloprid and trichlorfon), and commercial formulations of M. brunneum F52 (Met 52) containing only conidia. Field-collected grubs were susceptible to infection in a dosage-dependent relationship when exposed to potting soil treated with experimental microsclerotia granules in the laboratory. The LC50 for field-collected larvae was 14.2 mg of granules per cup (similar to 15 g granules/m(2)). Field plots treated with experimental and commercial formulations of M. brunneum F52 after 10 September (targeting second and third instar grubs) had significantly lower grub densities compared with untreated plots, providing 38.6-69.2% control, which sometimes equaled levels of control with chemical insecticides. Fungal treatments made prior to 21 August provided 14.3-69.3% control, although grub densities resulting from these treatments were often not significantly lower than those in untreated control plots. By comparison, chemical insecticide treatments provide 68-100% grub control, often providing better control when applied earlier in the season. In conclusion, P. japonica larvae are susceptible to infection by M. brunneum, and grub densities were reduced most consistently by fall applications targeting later instars.
机译：在实验室中和田间条件下，评价了Bernehizium brunneum（Petch）菌株F52的实验和商业制剂对日本甲虫，日本小新牛（Coleoptera：Scarbaeidae），幼虫（白色g）的控制作用。实验制剂由使用体外产生的菌核制成的颗粒和液体制剂组成，所述菌核的目的是在施用后产生感染性分生孢子孢子。将这些制剂与市售杀虫剂（吡虫啉和敌百虫）以及仅含分生孢子的商品布鲁氏杆菌F52（Met 52）进行了比较。当暴露于实验室中用实验性菌核颗粒处理的盆栽土壤中时，田间采集的g很容易受到剂量依赖性的感染。现场收集的幼虫的LC50为每杯14.2 mg颗粒（类似于15 g颗粒/ m（2））。 9月10日后，用实验和商业配方的布鲁氏杆菌F52处理的田间田地（针对第二龄和第三龄幼虫）与未处理田地相比，significantly密度显着降低，可提供38.6％至99.2％的控制，有时与化学杀虫剂的控制水平相当。 。 8月21日之前进行的真菌处理提供了14.3-69.3％的控制，尽管这些处理产生的g密度通常不会显着低于未处理的对照地块。相比之下，化学杀虫剂处理可提供68-100％的g控制，在季节较早时使用通常可提供更好的控制。总之，粳稻幼虫很容易受到布鲁氏杆菌的感染，而秋季施药针对幼虫后，g的密度最稳定地降低。

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