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首页> 外文期刊>Journal of Economic Entomology >Multiplex-PCR for Identification of Two Species in Genus Hishimonus (Hemiptera: Cicadellidae) in Jujube Orchards
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Multiplex-PCR for Identification of Two Species in Genus Hishimonus (Hemiptera: Cicadellidae) in Jujube Orchards

机译：多重PCR技术鉴定枣园中Hismonusus（Hemiptera：Cicadellidae）属的两种菌种

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摘要

The insect family Cicadellidae includes economically important vectors of plant pathogens. Hishimonus sellatus (Uhler) transmits jujube witches'-broom (JWB). Currently, H. sellatus and Hishimonus lamellatus Cai et Kuoh are observed to co-occur at the same locality on jujube. H. lamellatus is now suspected to be a JWB vector. As such, correct identification of Hishimonus species present in vineyards is essential for epidemiological surveys. However, traditional identification of Hishimonus by morphology is limited to the adult male. We provide a comprehensive description of morphological and molecular tools for discriminating between H. sellatus and H. lamellatus, for use in identification and monitoring of the two Hishimonus species and studies of their plant hosts. A rapid and inexpensive method is introduced to identify H. sellatus and H. lamellatus occurring in jujube orchards. This method is based on amplification of mitochondrial cytochrome oxidase I (COI) gene, using PCR with multiplexed, species-specific primers. The reliability of this new method has been tested on different populations from different sites in Beijing region of China.
机译：昆虫科（Cicadellidae）包括具有重要经济意义的植物病原体载体。 Hishimonus sellatus（Uhle​​r）传播枣巫婆的扫帚（JWB）。当前，观察到H. sellatus和Hismonus lamellatus Cai et Kuoh在枣的同一地点同时发生。现在怀疑嗜血杆菌是JWB载体。因此，正确识别葡萄园中存在的希氏菌属物种对于流行病学调查至关重要。然而，传统的通过形态学鉴定Hishimonus仅限于成年男性。我们提供了形态学和分子学工具的全面描述，用于区分蝶形嗜血杆菌和层状嗜血杆菌，用于鉴定和监测两个Hismonusus物种以及对其植物宿主的研究。引入了一种快速且廉价的方法来鉴定在枣园中发生的蝶鞍H.lamellatus。该方法基于使用多重物种特异性引物的PCR扩增线粒体细胞色素氧化酶I（COI）基因。该新方法的可靠性已在中国北京地区不同地点的不同人群中进行了测试。

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