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首页> 外文期刊>Journal of Economic Entomology >Two Chitin Biosynthesis Pathway Genes in Bactrocera dorsalis (Diptera: Tephritidae): Molecular Characteristics, Expression Patterns, and Roles in Larval-Pupal Transition
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Two Chitin Biosynthesis Pathway Genes in Bactrocera dorsalis (Diptera: Tephritidae): Molecular Characteristics, Expression Patterns, and Roles in Larval-Pupal Transition

机译：Bactrocera dorsalis（Diptera：Tephritidae）中的两个几丁质生物合成途径基因：分子特征，表达模式和在幼虫-up过渡中的作用

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Glucose-6-phosphate isomerase (G6PI) and UDP-N-acetylglucosamine pyrophosphorylase (UAP), two key components in the chitin biosynthesis pathway, are critical for insect growth and metamorphosis. In this study, we identified the genes BdG6PI and BdUAP from the oriental fruit fly, Bactrocera dorsalis (Hendel). The open reading frames (ORFs) of BdG6PI (1,491 bp) and BdUAP (1,677 bp) encoded 496 and 558 amino acid residues, respectively. Multiple sequence alignments showed that BdG6PI and BdUAP had high amino acid sequence identity with other insect homologues. Quantitative real-time polymerase chain reaction (qPCR) analysis indicated that BdG6PI was mainly expressed in the early stages of third-instar larvae and adults, while significantly higher expression of BdUAP was observed in adults. Both transcripts were expressed highly in the Malpighian tubules, but only slightly in the tracheae. The expression of both BdG6PI and BdUAP was significantly up-regulated by 20-hydroxyecdysone exposure and down-regulated by starvation. Moreover, injection of double-stranded RNAs of BdG6PI and BdUAP into third-instar larvae significantly reduced the corresponding gene expressions. Additionally, silencing of BdUAP resulted in 65% death and abnormal phenotypes of larvae, while silencing of BdG6PI had a slight effect on insect molting. These findings provide some data on the roles of BdG6PI and BdUAP in B. dorsalis and demonstrate the potential role for BdUAP in larval-pupal transition.
机译：几丁质生物合成途径中的两个关键成分是6-磷酸葡萄糖异构酶（G6PI）和UDP-N-乙酰氨基葡萄糖焦磷酸化酶（UAP），对昆虫的生长和变态至关重要。在这项研究中，我们从东方实蝇Bactrocera dorsalis（Hendel）鉴定了基因BdG6PI和BdUAP。 BdG6PI（1,491 bp）和BdUAP（1,677 bp）的开放阅读框（ORF）分别编码496和558个氨基酸残基。多个序列比对表明，BdG6PI和BdUAP与其他昆虫同源物具有高度氨基酸序列同一性。实时定量聚合酶链反应（qPCR）分析表明，BdG6PI主要在三龄幼虫和成虫的早期表达，而在成年人中观察到BdUAP的表达明显更高。两种转录本均在Malpighian小管中高表达，但在气管中仅轻度表达。 BdG6PI和BdUAP的表达均被20-羟基蜕皮激素暴露上调，而饥饿则下调。而且，将BdG6PI和BdUAP的双链RNA注射到三龄幼虫中会显着降低相应的基因表达。此外，BdUAP沉默导致65％的死亡和幼虫的异常表型，而BdG6PI沉默对昆虫的蜕皮有轻微影响。这些发现提供了有关BdG6PI和BdUAP在背侧芽孢杆菌中的作用的一些数据，并证明了BdUAP在幼虫-pu转化中的潜在作用。

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