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首页> 外文期刊>Journal of Economic Entomology >Effect of diatomaceous earth and Trichoderma harzianum T-22 (Rifai strain KRL-AG2) on the fungus gnat Bradysia sp nr. coprophila (Diptera : Sciaridae)
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Effect of diatomaceous earth and Trichoderma harzianum T-22 (Rifai strain KRL-AG2) on the fungus gnat Bradysia sp nr. coprophila (Diptera : Sciaridae)

机译：硅藻土和哈茨木霉T-22（Rifai菌株KRL-AG2）对真菌缓生菌的影响。亲（双翅目：：科）

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摘要

This study, consisting of three experiments, was designed to assess whether diatomaceous earth, when applied to the surface of growing media, reduces adult fungus gnat Bradysia sp. nr. coprophila (Diptera: Sciaridae) emergence or inhibits the females from laying eggs; and whether fungus gnat adults are attracted to the fungus Trichoderma harzianum T-22 (Rifai strain KRL-AG2) under laboratory conditions. In the first two experiments, diatomaceous earth was applied at two different thicknesses (3.1 and 6.3 mm) and conditions (dry and moist) to the surface of a growing medium (Universal SB 300 Mix) after the growing medium had been artificially inoculated with second or third instars of fungus gnats, or before female fungus gnat adults were released into each deli squat container. In the third experiment, preparations of the fungus T. harzianum at the highest recommended label rate (0.889 kg/m(3)) were amended into the growing medium and processed 24,48, or 72 h before use in a series of three two-choice trials with a two-armed experimental arena. In the first two experiments, the dry or moist layers of diatomaceous earth, in general, did not affect fungus gnats in terms of preventing adult emergence or egg laying by the females. During the course of these experiments, we observed that the diatomaceous earth dry treatments expanded as a result of absorbing moisture from the growing medium, creating fissures that allowed the fungus gnat larvae to pupate and females to lay eggs. In the third experiment, fungus gnat adults were not attracted to the T. harzianum treatments in any of the trials.
机译：该研究由三个实验组成，旨在评估硅藻土在应用于生长介质表面时是否能减少成年真菌Bradysia sp。 nr。亲蜂（双翅目：S虫科）出现或抑制雌性产卵;以及是否在实验室条件下将真菌成虫吸引到哈茨木霉T-22（日发菌株KRL-AG2）真菌上。在前两个实验中，在将生长培养基人工接种后，将硅藻土以两种不同的厚度（3.1和6.3毫米）和条件（干燥和湿润）施用于生长培养基（Universal SB 300 Mix）的表面或三龄真菌，或在雌性真菌成虫被放进每个熟食深蹲容器之前。在第三个实验中，将具有最高推荐标记率（0.889 kg / m（3））的哈茨木霉菌的制剂改良到生长培养基中，并在24,48或72小时内加工，然后以一系列三个三个在两手实验场上进行选择试验。在前两个实验中，硅藻土的干燥或潮湿层通常在防止成虫出现或雌性产卵方面不影响真菌。在这些实验过程中，我们观察到硅藻土的干燥处理由于吸收了生长介质中的水分而扩大，产生了裂痕，使真菌p幼虫化to，雌性产卵。在第三个实验中，在任何一项试验中，木耳成虫都没有被哈茨木霉的治疗所吸引。

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