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首页> 外文期刊>Journal of endotoxin research >CpG oligodeoxynucleotides stimulate IFN-gamma-inducible protein-10 production in human B cells.
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CpG oligodeoxynucleotides stimulate IFN-gamma-inducible protein-10 production in human B cells.

机译：CpG寡脱氧核苷酸可刺激人B细胞中IFN-γ诱导的蛋白10的产生。

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摘要

Several classes of CpG oligodeoxynucleotides (ODNs) with different immune stimulatory profiles were recently identified: the A-, B- and C-classes. In this study, we investigated the CpG-dependent stimulation of IFN-gamma-inducible protein 10 (IP-10 or CXCL10) in different human immune cell types. CpG ODNs induced IP-10 in monocytes, pDCs and in B cells. Purified B cells as well as RPMI 8226 cells responded to CpG stimulation by IP-10 production. Treatment with exogenous IFN-alpha2b sensitized PBMCs, purified B cells as well as RPMI 8226 cells to respond more efficiently to stimulation with CpG ODNs by IP-10 production. IP-10 signaling could be directly stimulated via TLR9 in CpG-unresponsive HEK293 cells transfected with human TLR9 and an IP-10 reporter construct. Therefore, CpG-mediated IP-10 production is stimulated through IFN-alpha in cells that express the IFN-alpha receptor, a second pathway for IP-10 induction exists in TLR9-expressing B cells and pDCs where IP-10 is stimulated directly upon CpG-mediated TLR9 signaling. Our data provide a better understanding of the mechanisms through which CpG ODNs induce efficient Th1 responses.
机译：最近发现了几类具有不同免疫刺激特征的CpG寡脱氧核苷酸（ODN）：A级，B级和C级。在这项研究中，我们研究了不同人类免疫细胞类型中CpG依赖性的IFN-γ诱导蛋白10（IP-10或CXCL10）的刺激。 CpG ODN在单核细胞，pDC和B细胞中诱导IP-10。纯化的B细胞以及RPMI 8226细胞通过IP-10产生对CpG刺激作出反应。用外源性IFN-α2b致敏的PBMC，纯化的B细胞以及RPMI 8226细胞进行处理，以更有效地响应IP-10生产对CpG ODN的刺激。 IP-10信号传导可通过TLR9直接刺激转染了人TLR9和IP-10报告基因构建体的CpG无反应性HEK293细胞。因此，在表达IFN-α受体的细胞中，CpG介导的IP-10的产生是通过IFN-α刺激的，在表达TLR9的B细胞和pDC中存在IP-10诱导的第二条途径，其中IP-10会直接受到刺激。 CpG介导的TLR9信号传导。我们的数据提供了对CpG ODN诱导有效Th1应答的机制的更好理解。

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