...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of entomology >Molecular Fingerprinting of Certain Cereal Aphids in Egypt (Hemiptera: Sternorrhyneha: Aphididae) Using RAPD and ISSRs Markers
【24h】

Molecular Fingerprinting of Certain Cereal Aphids in Egypt (Hemiptera: Sternorrhyneha: Aphididae) Using RAPD and ISSRs Markers

机译：使用RAPD和ISSRs标记对埃及某些谷物蚜虫（半翅目：Sternorrhyneha：Aphididae）进行分子指纹分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cereal aphids are one of the most important insect pests limiting cereal production worldwide. Classical morphological criteria for aphid species identification may be affected by environmental factors such as climatic conditions and physiological status of the host plant. So, two modern molecular techniques; Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSRs) were used to find diagnostic markers for fingerprinting eleven cereal aphids those collected from different cereal plants and from different localities in Egypt. Eight RAPD and five ISSRs primers were successfully produced 97 and 69 markers that could be used to differentiate the eleven different cereal aphid species. Also these molecular techniques with 23 diagnostic morphological characters were used to find the Phylogenetic relationship among the different collected species; that divided into two clusters with similarity matrix percentages of 73 and 82%. From these results it could concluded that these techniques could be successfully used successively to fingerprint and identify these aphid species and differentiate among them.
机译：谷物蚜虫是限制全世界谷物生产的最重要的害虫之一。蚜虫物种鉴定的经典形态学标准可能会受到环境因素的影响，例如气候条件和寄主植物的生理状况。因此，两种现代分子技术;随机扩增多态性DNA（RAPD）和内部简单序列重复（ISSR）用于寻找指纹图谱，以鉴定11种谷物蚜虫的指纹，这些蚜虫是从埃及不同谷物植物和不同地区收集的。成功产生了八种RAPD和五种ISSRs引物，可用于区分11种不同的谷物蚜虫物种的97和69个标记。这些具有23种形态学特征的分子技术也被用来发现不同物种之间的亲缘关系。分为两个相似度百分比分别为73％和82％的聚类。从这些结果可以得出结论，这些技术可以成功地连续用于指纹鉴定和鉴定这些蚜虫种类并在它们之间进行区分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号