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Production of soluble microbial products (SMP) in anaerobic chemostats under nutrient deficiency

机译:营养缺乏时厌氧型恒化器中可溶性微生物产物(SMP)的产生

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It is known that in well-operated anaerobic systems the majority of the residual chemical oxygen demand (COD) present in the effluent is due to soluble microbial products (SMP) generated during treatment. SNIP production is affected by many factors, and this study investigated the effect of nutrient deficiency on SMP production in anaerobic chemostats fed on glucose as the sole carbon source. The results showed that under steady-state conditions the SMP/CODout ratio was as high as 95% and the normalized SMP production (SMP/S-o) averaged 3%. During nutrient deficiency the SMP/CODout ratio averaged 45% indicating that the importance of SMP in the effluent was reduced due to the enhanced production of VFAs. Nevertheless, under such stressed conditions the normalized production of SMP (SMP/S-o) increased reaching up to 37%. Analysis of extracellular polymers (ECP) indicated that ECPc production was enhanced after nutrient cessation and chemical analyses of the effluent suggest that most of the SMP was not protein- or carbohydrate-like material. DNA analysis indicates that part of the SMP produced during nutrient deficiency might be due to enhanced cell lysis, although some organics might have been deliberately excreted to scavenge metal nutrients. Biochemical methane potential (BMP) assays showed that the lack of N and P resulted in the highest SMP production which might have been released to dump electrons that could not be used in cell synthesis due to the lack of these macronutrients. [References: 32]
机译:众所周知,在运行良好的厌氧系统中,废水中存在的大多数残余化学需氧量(COD)是由于处理过程中产生的可溶性微生物产物(SMP)引起的。 SNIP的产生受多种因素影响,本研究调查了营养不足对以葡萄糖为唯一碳源的厌氧型恒化器中SMP产生的影响。结果表明,在稳态条件下,SMP / CODout比例高达95%,标准化SMP产量(SMP / S-o)平均为3%。在养分缺乏期间,SMP / CODout比率平均为45%,这表明由于VFA产量的增加,SMP在废水中的重要性降低了。但是,在这种压力条件下,SMP的标准化产量(SMP / S-o)增加到高达37%。细胞外聚合物(ECP)的分析表明,养分停止后,ECPc的产量增加了,废水的化学分析表明,大多数SMP不是蛋白质或碳水化合物样物质。 DNA分析表明,养分缺乏期间产生的部分SMP可能是由于细胞溶解增强所致,尽管某些有机物可能被故意排泄以清除金属养分。甲烷生物化学势(BMP)分析表明,氮和磷的缺乏导致最高的SMP产生,由于缺乏这些大量营养素,SMP的产生可能被释放到电子合成中,而这些电子无法用于细胞合成。 [参考:32]

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