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首页> 外文期刊>Journal of enzyme inhibition >Non-enzymatic glycosylation (or glycation) and inhibition of the pig heart cytosolic aspartate aminotransferase by glyceraldehyde 3-phosphate.
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Non-enzymatic glycosylation (or glycation) and inhibition of the pig heart cytosolic aspartate aminotransferase by glyceraldehyde 3-phosphate.

机译：非酶糖基化（或糖基化）和3-磷酸甘油醛对猪心脏胞质天冬氨酸转氨酶的抑制作用。

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摘要

Glyceraldehyde 3-phosphate (Glyc3P), a glycolytic intermediate, non-enzymatically glycosylated (or glycated) and inhibited the pig heart cytoplasmic aspartate aminotransferase (cAAT). Glyc3P (5.0 mM) decreased cAAT activity by 47% after 1 min at 23 degrees C. cAAT activity remained unchanged after a 24h incubation with either glucose 6-phosphate (5.0 mM) or ribose 5-phosphate (5.0 mM). Increasing the incubation pH from 6.4 to 7.8 or the incubation temperature from 23 degrees C to 50 degrees C enhanced Glyc3P's inhibitory effect on cAAT activity. Glyc3P (250-500 microM) decreased the thermal stability of cAAT as evidenced by lowering the Tm or temperature that caused a 50% irreversible loss of cAAT activity (69 degrees C, control; 58.5 degrees C, 500 microM Glyc3P). Glyc3P decreased cAAT amino group content and increased glycation products, which were measured by adduct formation, fluorescence and protein crosslinking.
机译：3-磷酸​​甘油醛（Glyc3P），一种糖酵解中间体，非酶促糖基化（或糖基化），可抑制猪心脏的细胞质天冬氨酸转氨酶（cAAT）。 Glyc3P（5.0 mM）在23摄氏度下1分钟后，cAAT活性降低了47％。与6磷酸葡萄糖（5.0 mM）或5磷酸核糖（5.0 mM）孵育24小时后，cAAT活性保持不变。将孵育pH从6.4增加到7.8或将孵育温度从23摄氏度增加到50摄氏度，可以增强Glyc3P对cAAT活性的抑制作用。 Glyc3P（250-500 microM）降低了cAAT的热稳定性，这是由于降低了导致50％的cAAT活性不可逆损失的Tm或温度降低的（69摄氏度，对照; 58.5摄氏度，500 microM Glyc3P）。 Glyc3P降低了cAAT氨基含量并增加了糖基化产物，这可以通过加合物形成，荧光和蛋白质交联来测量。

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