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首页> 外文期刊>Journal of Experimental Botany >Altered stomatal dynamics in ascorbate oxidase over-expressing tobacco plants suggest a role for dehydroascorbate signalling
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Altered stomatal dynamics in ascorbate oxidase over-expressing tobacco plants suggest a role for dehydroascorbate signalling

机译：过表达抗坏血酸氧化酶的烟草植物气孔动力学变化表明脱氢抗坏血酸信号的作用

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摘要

Control of stomatal aperture is of paramount importance for plant adaptation to the surrounding environment. Here, we report on several parameters related to stomatal dynamics and performance in transgenic tobacco plants (Nicotiana tabacum L., cv. Xanthi) over-expressing cucumber ascorbate oxidase (AO), a cell wall-localized enzyme of uncertain biological function that oxidizes ascorbic acid (AA) to monodehydroascorbic acid which dismutates yielding AA and dehydroascorbic acid (DHA). In comparison to WT plants, leaves of AO over-expressing plants exhibited reduced stomatal conductance (due to partial stomatal closure), higher water content, and reduced rates of water loss on detachment. Transgenic plants also exhibited elevated levels of hydrogen peroxide and a decline in hydrogen peroxide-scavenging enzyme activity. Leaf ABA content was also higher in AO over-expressing plants. Treatment of epidermal strips with either 1 mM DHA or 100 mu M hydrogen peroxide resulted in rapid stomatal closure in WT plants, but not in AO-over-expressing plants. This suggests that signal perception and/or transduction associated with stomatal closure is altered by AO over-expression. These data support a specific role for cell wall-localized AA in the perception of environmental cues, and suggest that DHA acts as a regulator of stomatal dynamics.
机译：气孔孔径的控制对于植物适应周围环境至关重要。在这里，我们报告了与过量表达黄瓜抗坏血酸氧化酶（AO）的转基因烟草植物（Nicotiana tabacum L.，cv。Xanthi）气孔动态和性能相关的几个参数，这是一种细胞壁定位的不确定生物功能的酶，可氧化抗坏血酸。酸（AA）转变成一元脱氢抗坏血酸，从而脱去AA和脱氢抗坏血酸（DHA）。与野生型植物相比，过量表达AO的植物的叶片表现出降低的气孔导度（由于部分气孔关闭），更高的水分含量以及减少的水流失率。转基因植物还表现出过氧化氢水平升高和过氧化氢清除酶活性下降。在AO过表达植物中，叶片ABA含量也更高。用1 mM DHA或100μM过氧化氢处理表皮条在WT植物中导致气孔快速闭合，但在AO过表达植物中却没有。这表明与气孔关闭相关的信号感知和/或转导因AO过表达而改变。这些数据支持在环境线索的感知中细胞壁定位的AA的特定作用，并暗示DHA充当气孔动力学的调节剂。

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