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首页> 外文期刊>Journal of Experimental Botany >Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat
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Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat

机译：通过bZIP型转录因子LIP19在普通小麦中产生非生物胁迫耐受性

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Cereal lip19 genes encoding bZIP-type transcription factors are assumed to play a regulatory role in gene expression during the cold acclimation process. However, no direct evidence shows an association of LIP19-type bZIPs with stress tolerance or activation of stress-responsive Cor/Lea genes. To understand the molecular basis of development of abiotic stress tolerance through the LIP19 transcription factor, a wheat lip19 homologue, Wlip19, was isolated and characterized. Wlip19 expression was activated by low temperature in seedlings and was higher in a freezing-tolerant cultivar than in a freezing-sensitive one. Wlip19 also responded to drought and exogenous ABA treatment. Wlip19-expressing transgenic tobacco showed a significant increase in abiotic stress tolerance, especially freezing tolerance. Expression of a GUS reporter gene under the control of promoter sequences of four wheat Cor/Lea genes, Wdhn13, Wrab17, Wrab18, and Wrab19, was enhanced by Wlip19 expression in wheat callus and tobacco plants. These results indicate that WLIP19 acts as a transcriptional regulator of Cor/Lea genes in the development of abiotic stress tolerance. Moreover, direct protein-protein interaction between WLIP19 and a wheat OBF1 homologue TaOBF1, another bZIP-type transcription factor, was observed, suggesting that this interaction is conserved in cereals.
机译：假定在寒冷的适应过程中，编码bZIP型转录因子的谷物lip19基因在基因表达中起调节作用。但是，没有直接的证据显示LIP19型bZIP与应激耐受性或应激反应性Cor / Lea基因的激活有关。为了了解通过LIP19转录因子发展非生物胁迫耐受性的分子基础，分离并鉴定了小麦lip19同源物Wlip19。 Wlip19的表达被幼苗中的低温激活，并且在耐冻品种中比在对冷冻敏感的品种中更高。 Wlip19还对干旱和外源ABA处理产生了反应。表达Wlip19的转基因烟草显示出非生物胁迫耐受性，特别是冷冻耐受性的显着提高。在四个小麦Cor / Lea基因Wdhn13，Wrab17，Wrab18和Wrab19的启动子序列控制下，GUS报告基因的表达因Wlip19在小麦愈伤组织和烟草植物中的表达而增强。这些结果表明WLIP19在非生物胁迫耐受性的发展中充当Cor / Lea基因的转录调节剂。此外，观察到WLIP19与小麦OBF1同源TaOBF1（另一种bZIP型转录因子）之间存在直接的蛋白质-蛋白质相互作用，这表明这种相互作用在谷物中得以保留。

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