...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Molecular cloning and characterization of a salinity stress-induced gene encoding DEAD-box helicase from the halophyte Apocynum venetum
【24h】

Molecular cloning and characterization of a salinity stress-induced gene encoding DEAD-box helicase from the halophyte Apocynum venetum

机译：盐生植物罗布麻盐渍胁迫诱导DEAD-box解旋酶编码基因的分子克隆与表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The genes encoding DEAD-box helicases play a key role in various abiotic stresses, including temperature, light, oxygen, and salt stress. A salt-responsive gene, designated AvDH1, was isolated from the halophyte dogbane (Apocynum venetum) by using suppression subtractive hybridization and RACE (rapid amplification of cDNA ends) PCR. The deduced amino acid sequence has nine conserved helicase motifs of the DEAD-box protein family. The AvDH1 gene is present as a single copy in the dogbane genome. This gene is expressed in response to NaCl and not polyethlene glycol (PEG) nor abscisic acid, and its expression increases with time. The transcription of AvDH1 is also induced by low temperature (4 degrees C), but its accumulation first increases then decreases with time. The purified recombinant protein contains ATP-dependent DNA helicase activity, ATP-independent RNA helicase activity, and DNA- or RNA-dependent ATPase activity. The ATPase activity of AvDH1 is stimulated more by single-stranded DNA than by double-stranded DNA or RNA. These results suggested that AvDH1 belonging to the DEAD-box helicase family is induced by salinity, functions as a typical helicase to unwind DNA and RNA, and may play an important role in salinity tolerance.
机译：编码DEAD-box解旋酶的基因在各种非生物胁迫（包括温度，光照，氧气和盐胁迫）中起关键作用。通过使用抑制性消减杂交和RACE（cDNA末端的快速扩增）PCR，从盐生植物的狗b（罗布麻）中分离出一种盐响应基因，称为AvDH1。推导的氨基酸序列具有DEAD-box蛋白家族的9个保守的解旋酶基序。 AvDH1基因在犬型基因组中以单拷贝形式存在。该基因是响应NaCl而表达的，而不是聚乙二醇（PEG）或脱落酸表达的，并且其表达随时间增加。 AvDH1的转录也由低温（4摄氏度）诱导，但其积累先增加后随时间减少。纯化的重组蛋白包含ATP依赖性DNA解旋酶活性，ATP依赖性RNA解旋酶活性以及DNA或RNA依赖性ATPase活性。与单链DNA或RNA相比，单链DNA对AvDH1的ATPase活性的刺激更大。这些结果表明，属于DEAD-box解旋酶家族的AvDH1受盐度诱导，起典型的解旋酶作用，解链DNA和RNA，并可能在盐度耐受中起重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号