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首页> 外文期刊>Journal of Experimental Botany >Activity of tonoplast proton pumps and Na/H exchange in potato cell cultures is modulated by salt.
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Activity of tonoplast proton pumps and Na/H exchange in potato cell cultures is modulated by salt.

机译：盐调节马铃薯细胞培养液中质子泵质子泵的活性和Na / H交换。

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The efficient exclusion of excess Na from the cytoplasm and vacuolar Na accumulation are the main mechanisms for the adaptation of plants to salt stress. This is typically carried out by transmembrane transport proteins that exclude Na from the cytosol in exchange for H, a secondary transport process which is energy-dependent and driven by the proton-motive force generated by plasma-membrane and tonoplast proton pumps. Tonoplast enriched-vesicles from control and 150 mM NaCl-tolerant calli lines were used as a model system to study the activity of V-H-PPase and V-H-ATPase and the involvement of Na compartmentalization into the vacuole as a mechanism of salt tolerance in Solanum tuberosum. Both ATP- and pyrophosphate (PPi)-dependent H-transport were higher in tonoplast vesicles from the salt-tolerant line than in vesicles from control cells. Western blotting of tonoplast proteins confirmed that changes in V-H-PPase activity are correlated with increased protein amount. Conversely, immunodetection of the A-subunit of V-H-ATPase revealed that a mechanism of post-translational regulation is probably involved. Na-dependent dissipation of a pre-established pH gradient was used to measure Na/H exchange in tonoplast vesicles. The initial rates of proton efflux followed Michaelis-Menten kinetics and the Vmax of proton dissipation was 2-fold higher in NaCl-tolerant calli when compared to the control. H-coupled exchange was specific for Na and Li and not for K. The increase of both the pH gradient across the tonoplast and the Na/H antiport activity in response to salt strongly suggests that Na sequestration into the vacuole contributes to salt tolerance in potato.
机译：有效地从细胞质中排除过量的Na和液泡状的Na积累是植物适应盐胁迫的主要机制。这通常是通过跨膜转运蛋白来完成的，该跨膜转运蛋白将Na从细胞质中排除，以换取H，这是一个次级转运过程，其依赖于能量，并由质膜和液泡质子泵产生的质子动力驱动。使用来自对照和150 mM NaCl耐性愈伤组织的液泡膜富集囊泡作为模型系统，研究VH-PPase和VH-ATPase的活性以及Na分隔进入液泡作为茄子中耐盐性的机制。耐盐品系的液泡膜囊泡中的ATP和焦磷酸（PPi）依赖性H转运均高于对照细胞的囊泡。液泡膜蛋白的Western印迹证实，V-H-PPase活性的变化与蛋白量增加有关。相反，对V-H-ATPase A亚基的免疫检测表明可能涉及翻译后调控机制。预先建立的pH梯度的Na依赖性消散用于测量液泡膜囊泡中的Na / H交换。质子外排的初始速率遵循Michaelis-Menten动力学，耐NaCl的愈伤组织中质子耗散的Vmax比对照高2倍。 H耦合的交换对Na和Li而不是对K特异。液泡中pH梯度和响应盐分的Na / H反向转运活性的增加都强烈表明，将Na隔离到液泡中有助于马铃薯的耐盐性。

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