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首页> 外文期刊>Journal of Experimental Botany >Effects of o-1,3-glucan from Septoria tritici on structural defence responses in wheat.
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Effects of o-1,3-glucan from Septoria tritici on structural defence responses in wheat.

机译：小麦白僵菌中o-1,3-葡聚糖对小麦结构防御反应的影响。

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摘要

The accumulation of the pathogenesis-related (PR) proteins beta-1,3-glucanase and chitinase and structural defence responses were studied in leaves of wheat either resistant or susceptible to the hemibiotrophic pathogen Septoria tritici. Resistance was associated with an early accumulation of beta-1,3-glucanase and chitinase transcripts followed by a subsequent reduction in level. Resistance was also associated with high activity of beta-1,3-glucanase, especially in the apoplastic fluid, in accordance with the biotrophic/endophytic lifestyle of the pathogen in the apoplastic spaces, thus showing the highly localized accumulation of defence proteins in the vicinity of the pathogen. Isoform analysis of beta-1,3-glucanase from the apoplastic fluid revealed that resistance was associated with the accumulation of an endo-beta-1,3-glucanase, previously implicated in defence against pathogens, and a protein with identity to ADPG pyrophosphatase (92%) and germin-like proteins (93%), which may be involved in cell wall reinforcement. In accordance with this, glycoproteins like extensin were released into the apoplast and callose accumulated to a greater extent in cell walls, whereas lignin and polyphenolics were not found to correlate with defence. Treatment of a susceptible wheat cultivar with purified beta-1,3-glucan fragments from cell walls of S. tritici gave complete protection against disease and this was accompanied by increased gene expression of beta-1,3-glucanase and the deposition of callose. Collectively, these data indicate that resistance is dependent on a fast, initial recognition of the pathogen, probably due to beta-1,3-glucan in the fungal cell walls, and this results in the accumulation of beta-1,3-glucanase and structural defence responses, which may directly inhibit the pathogen and protect the host against fungal enzymes and toxins.
机译：研究了与病原相关的（PR）蛋白β-1,3-葡聚糖酶和几丁质酶的积累以及结构防御反应，这些小麦叶片对半养营养性病原体小麦枯萎病具有抗性或易感性。耐药性与β-1,3-葡聚糖酶和几丁质酶转录物的早期积累有关，随后水平降低。抗药性还与β-1,3-葡聚糖酶的高活性有关，尤其是在质外体液中，这取决于病原体在质外体空间中的营养性/内生性生活方式，因此显示了防御蛋白在附近的高度局部性积累病原体。从质外体液体中对β-1,3-葡聚糖酶的同工型分析表明，耐药性与内在的β-1,3-葡聚糖酶的积累有关，该酶以前与病原体的防御有关，并且与ADPG焦磷酸酶具有相同的蛋白质（ 92％）和胚芽蛋白样蛋白（93％），它们可能参与细胞壁增强。据此，诸如延伸蛋白之类的糖蛋白被释放到质外体中并且愈伤组织在细胞壁中积累的程度更大，而木质素和多酚类化合物却与防御能力无关。用来自小麦链霉菌细胞壁的纯化的β-1,3-葡聚糖片段处理易感小麦品种，可完全防御疾病，并伴有β-1,3-葡聚糖酶基因表达增加和call质沉积。总体而言，这些数据表明耐药性取决于病原体的快速初始识别，这可能是由于真菌细胞壁中的β-1,3-葡聚糖引起的，这导致β-1,3-葡聚糖酶和结构防御反应，可以直接抑制病原体并保护宿主抵抗真菌酶和毒素。

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