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首页> 外文期刊>Journal of Experimental Botany >Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicae and oligophagous Brevicoryne brassicae
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Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicae and oligophagous Brevicoryne brassicae

机译：多食性Myzus persicae和少食性Brevicoryne brasicae攻击后，具有不同芥子油苷特征的拟南芥生态型的转录响应

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Plants are equipped with a range of defence mechanisms against herbivorous insects. In cruciferous species, jasmonic acid, salicylic acid, and ethylene along with glucosinolates and their hydrolysis products play important roles in plant protection and plant-insect communication. In turn, a number of herbivores have adapted to plants that contain glucosinolates. As a result of adaptation to their host plants, specialized insects may elicit different plant-inducible responses than generalists. Oligonucleotide microarrays and qRT-PCR analysis were used to characterize transcriptional profiles of Arabidopsis thaliana plants in response to infestation with a generalist aphid, Myzus persicae, or a cruciferous plant specialist, Brevicoryne brassicae. To find possible differences and similarities in molecular responses between plants differing in predominant glucosinolate hydrolysis products, three ecotypes of A. thaliana were chosen: Wassilewskija (Ws), Cape Verde Islands (Cvi), and Landsberg erecta (Ler), which, respectively, produce mainly isothiocyanates, epithionitriles, and nitriles. In all three ecotypes, general stress-responsive genes, genes belonging to octadecanoid and indole glucosinolate synthesis pathways were induced upon both generalist and specialist attack. By contrast, transcription of myrosinases, enzymes hydrolysing glucosinolates, was suppressed. The induction of the jasmonic acid synthesis pathway was strongest in Cvi, while the up-regulation of the indole glucosinolate synthesis pathway was highest in Ler, suggesting a slightly different defence strategy in these two ecotypes. Specialist and generalist infestations caused statistically significant differential regulation of 60 genes in Ws and 21 in Cvi. Among these were jasmonic acid and tryptophan synthesis pathway enzymes, and pathogenesis related protein (PR1). Insect no-choice experiments revealed lowered fitness of B. brassicae on Ler and Cvi in comparison to Ws, but no ecotype-dependent change in fecundity of M. persicae. Targeted studies employing constructs of GUS reporter gene under the control of promoters from CYP79B2 and CYP79B3 genes showed insect-specific induction of the indole glucosinolates synthesis pathway.
机译：植物配备了一系列针对草食性昆虫的防御机制。在十字花科物种中，茉莉酸，水杨酸和乙烯以及芥子油苷及其水解产物在植物保护和植物-昆虫传播中起着重要作用。反过来，许多草食动物已经适应了含有芥子油苷的植物。由于适应了它们的寄主植物，专门昆虫可能引起与普通昆虫不同的植物诱导反应。寡核苷酸微阵列和qRT-PCR分析被用来表征拟南芥植物的转录谱，以应对全能蚜虫Myzus persicae或十字花科植物Brevicoryne Brassicae的侵染。为了发现在主要硫代芥子油苷水解产物不同的植物之间的分子反应之间可能存在的差异和相似之处，选择了三种拟南芥生态型：Wassilewskija（Ws），佛得角群岛（Cvi）和Landsberg erecta（Ler），主要生产异硫氰酸盐，表硫腈和腈。在这三种生态型中，普通攻击和专家攻击都诱导了一般的应激反应基因，属于十八烷类和吲哚芥子油苷合成途径的基因。相反，抑制了芥子油苷酶（水解芥子油苷的酶）的转录。茉莉酸合成途径的诱导在Cvi中最强，而吲哚芥子油苷合成途径的上调在Ler中最高，表明这两种生态型的防御策略略有不同。专家和普通病患导致Ws中60个基因和Cvi中21个基因的统计学差异显着。其中包括茉莉酸和色氨酸合成途径酶，以及发病相关蛋白（PR1）。昆虫无选择实验显示，与Ws相比，B。brasicae在Ler和Cvi上的适应性降低，但没有生态型依赖性的M. persicae繁殖力变化。在来自CYP79B2和CYP79B3基因启动子的控制下，采用GUS报告基因构建体的靶向研究显示，昆虫特异性诱导吲哚芥子油苷合成途径。

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