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首页> 外文期刊>Journal of Experimental Botany >Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens
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Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens

机译：金属硫蛋白2和3有助于金属适应的表型，但不与金属超蓄积藻（Thlaspi caerulescens）中的锌积累直接相关。

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摘要

To study the role of metallothioneins (MTs) in Zn accumulation, the expression of TcMT2a, TcMT2b, and TcMT3 was analysed in three accessions and 15 F(3) families of two inter-accession crosses of the Cd/Zn hyperaccumulator Thlaspi caerulescens, with different degrees of Zn accumulation. The highest expression levels were found in the shoots of a superior metal-accumulating calamine accession from St Laurent le Minier, with >10-fold TcMT3 expression compared with another calamine accession and a non-metallicolous accession. Moreover, F(3) sibling lines from the inter-accession crosses that harboured the MT2a or MT3 allele from St Laurent le Minier had higher expression levels. However, there was no co-segregation of TcMT2a or TcMT3 expression and Zn accumulation. To examine the functions of TcMTs in plants, TcMT2a and TcMT3 were ectopically expressed in Arabidopsis. The transformant lines had reduced root length in control medium but not at high metal concentrations, suggesting that the ectopically expressed proteins interfered with the physiological availability of essential metals under limited supply. The Arabidopsis transformant lines did not show increased tolerance to Cd, Cu, or Zn, nor increased Cd or Zn accumulation. Immunohistochemical analysis indicated that in roots, MT2 protein is localized in the epidermis and root hairs of both T. caerulescens and Arabidopsis thaliana. The results suggest that TcMT2a, TcMT2b, and TcMT3 are not primarily involved in Zn accumulation as such. However, the elevated expression levels in the metallicolous accessions suggests that they do contribute to the metal-adapted phenotype, possibly through improving Cu homeostasis at high Zn and Cd body burdens. Alternatively, they might function as hypostatic enhancers of Zn or Cd tolerance.
机译：若要研究金属硫蛋白（MTs）在锌积累中的作用，分析了Cd / Zn超富集性拟南芥（Thlaspi caerulescens）的三个种质和15 F（3）族的两个种质间杂交的TcMT2a，TcMT2b和TcMT3的表达，不同程度的锌积累。在来自圣洛朗（Le Laurent le Minier）的优质金属富集甘氨酰胺登录芽的芽中发现了最高的表达水平，与另一种甘氨酰胺登录和非金属的登录登录相比，TcMT3表达> 10倍。此外，来自藏有圣罗兰特米勒的MT2a或MT3等位基因的种间杂交的F（3）同系具有更高的表达水平。但是，没有TcMT2a或TcMT3表达和锌积累的共同分离。为了检查植物中TcMT的功能，拟南芥中异位表达了TcMT2a和TcMT3。转化株在控制培养基中的根长减少了，但在高金属浓度下却没有，表明异位表达的蛋白质在供应有限的情况下干扰了必需金属的生理可用性。拟南芥转化株没有显示出对Cd，Cu或Zn的耐受性增加，也没有显示Cd或Zn积累的增加。免疫组织化学分析表明，在根中，MT2蛋白位于青枯菌和拟南芥的表皮和根毛中。结果表明，TcMT2a，TcMT2b和TcMT3本身并不主要参与锌的积累。但是，金属质种质中表达水平的升高表明它们确实有助于金属适应表型，可能是通过改善高锌和镉人体负荷下的铜稳态而实现的。或者，它们可能充当锌或镉耐受性的抑制性增强剂。

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