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首页> 外文期刊>Journal of Experimental Botany >Proteome changes induced by aluminium stress in tomato roots
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Proteome changes induced by aluminium stress in tomato roots

机译：铝胁迫下番茄根中蛋白质组的变化

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摘要

Growth inhibition in acid soils due to Al stress affects crop production worldwide. To understand mechanisms in sensitive crops that are affected by Al stress, a proteomic analysis of primary tomato root tissue, grown in Al-amended and non-amended liquid cultures, was performed. DIGE-SDS-MALDI-TOF-TOF analysis of these tissues resulted in the identification of 49 proteins that were differentially accumulated. Dehydroascorbate reductase, glutathione reductase, and catalase enzymes associated with antioxidant activities were induced in Al-treated roots. Induced enzyme proteins associated with detoxification were mitochondrial aldehyde dehydrogenase, catechol oxidase, quinone reductase, and lactoylglutathione lyase. The germin-like (oxalate oxidase) proteins, the malate dehydrogenase, wali7 and heavy-metal associated domain-containing proteins were suppressed. VHA-ATP that encodes for the catalytic subunit A of the vacuolar ATP synthase was induced and two ATPase subunit 1 isoforms were suppressed. Several proteins in the active methyl cycle, including SAMS, quercetin 3-O-methyltransferase and AdoHcyase, were induced by Al stress. Other induced proteins were isovaleryl-CoA dehydrogenase and the GDSL-motif lipase hydrolase family protein. NADPH-dependent flavin reductase and beta-hydroxyacyl-ACP dehydratase were suppressed.
机译：由于铝胁迫而在酸性土壤中的生长抑制影响了全世界的农作物产量。为了了解受铝胁迫影响的敏感作物的机理，对在铝改良和非改良液体培养基中生长的番茄根部组织进行了蛋白质组学分析。对这些组织进行DIGE-SDS-MALDI-TOF-TOF分析可鉴定出49种差异积累的蛋白质。在铝处理过的根中诱导了与抗氧化活性相关的脱氢抗坏血酸还原酶，谷胱甘肽还原酶和过氧化氢酶。与排毒有关的诱导酶蛋白是线粒体醛脱氢酶，儿茶酚氧化酶，醌还原酶和乳酰谷胱甘肽裂解酶。抑制了胚芽状蛋白（草酸氧化酶），苹果酸脱氢酶，wali7和重金属相关的含域蛋白。诱导编码液泡ATP合酶催化亚基A的VHA-ATP，并抑制了两个ATPase亚基1亚型。 Al胁迫诱导了活跃的甲基循环中的几种蛋白质，包括SAMS，槲皮素3-O-甲基转移酶和AdoHcyase。其他诱导的蛋白质是异戊酰-CoA脱氢酶和GDSL-基序脂肪酶水解酶家族蛋白。 NADPH依赖的黄素还原酶和β-羟酰基ACP脱水酶被抑制。

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