...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Arabidopsis thaliana POLYOL/MONOSACCHARIDE TRANSPORTERS 1 and 2: fructose and xylitol/H+ symporters in pollen and young xylem cells.
【24h】

Arabidopsis thaliana POLYOL/MONOSACCHARIDE TRANSPORTERS 1 and 2: fructose and xylitol/H+ symporters in pollen and young xylem cells.

机译：花粉和木质部年轻细胞中的拟南芥多元醇/单糖转运蛋白1和2：果糖和木糖醇/ H + 转运蛋白。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The genome of Arabidopsis thaliana contains six genes, AtPMT1 to AtPMT6 (Arabidopsis thaliana POLYOL/MONOSACCHARIDE TRANSPORTER 1-6), which form a distinct subfamily within the large family of more than 50 monosaccharide transporter-like (MST-like) genes. So far, only AtPMT5 [formerly named AtPLT5 (At3 g18830)] has been characterized and was shown to be a plasma membrane-localized H+-symporter with broad substrate specificity. The characterization of AtPMT1 (At2 g16120) and AtPMT2 (At2 g16130), two other, almost identical, members of this transporter subfamily, are presented here. Expression of the AtPMT1 and AtPMT2 cDNAs in baker's yeast (Saccharomyces cerevisiae) revealed that these proteins catalyse the energy-dependent, high-capacity transport of fructose and xylitol, and the transport of several other compounds with lower rates. Expression of their cRNAs in Xenopus laevis oocytes showed that both proteins are voltage-dependent and catalyse the symport of their substrates with protons. Fusions of AtPMT1 or AtPMT2 with the green fluorescent protein (GFP) localized to Arabidopsis plasma membranes. Analyses of reporter genes performed with AtPMT1 or AtPMT2 promoter sequences showed expression in mature (AtPMT2) or germinating (AtPMT1) pollen grains, as well as in growing pollen tubes, hydathodes, and young xylem cells (both genes). The expression was confirmed with an anti-AtPMT1/AtPMT2 antiserum ( alpha AtPMT1/2) raised against peptides conserved in AtPMT1 and AtPMT2. The physiological roles of the proteins are discussed and related to plant cell wall modifications.
机译：拟南芥的基因组包含六个基因， AtPMT1 至 AtPMT6 （拟南芥POLYOL / MONOSACCHARIDE TRANSPORTER 1-6 ），在超过50个单糖转运蛋白样（MST样）基因的大家族中形成一个独特的亚家族。到目前为止，只有AtPMT5 [以前称为AtPLT5（At3 g18830）]得以表征，并显示为具有广泛底物特异性的质膜定位H + 转运体。此处介绍了该转运蛋白亚家族的另外两个几乎相同的成员AtPMT1（At2 g16120）和AtPMT2（At2 g16130）的表征。 AtPMT1 和 AtPMT2 cDNA在面包酵母（ Saccharomyces cerevisiae ）中的表达表明，这些蛋白质催化能量依赖的高容量转运果糖和木糖醇，以及其他几种速率较低的化合物的运输。它们的cRNA在非洲爪蟾卵母细胞中的表达表明，这两种蛋白都是电压依赖性的，并催化质子与底物的共生。 AtPMT1或AtPMT2与绿色荧光蛋白（GFP）的融合物位于拟南芥（arabidopsis）质膜上。用 AtPMT1 或 AtPMT2 启动子序列进行的报告基因分析显示在成熟（ AtPMT2 ）或发芽（ AtPMT1 ）花粉粒，以及正在生长的花粉管，水da和幼木质部细胞（均为基因）。用针对AtPMT1和AtPMT2中保守的肽的抗AtPMT1 / AtPMT2抗血清（αAtPMT1 / 2）证实了该表达。讨论了蛋白质的生理作用，并与植物细胞壁修饰有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号