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首页> 外文期刊>Journal of Experimental Botany >Silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid.
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Silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid.

机译：水飞蓟素的细胞培养物中茉莉酸甲酯的分泌和水飞蓟素的分泌是由磷脂酶D-磷脂酸介导的。

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摘要

The flavonolignan silymarin is released to the extracellular medium of Silybum marianum cultures and its production can be stimulated by the elicitor methyljasmonate (MeJA). The sequence of the signalling processes leading to this response is unknown at present. It is reported in this work that MeJA increased the activity of the enzyme phospholipase D (PLD). Treatment with mastoparan (Mst), a PLD activity stimulator, also enhanced PLD and caused a substantial increase in silymarin production. The application of the product of PLD activity, phosphatidic acid (PA) promoted silymarin accumulation. Altering PLD activity by introducing in cultures n-butanol (nBuOH), which inhibits PA production by PLD, prevented silymarin elicitation by MeJA or Mst and also impeded its release in non-elicited cultures. Treatment with iso-, sec- or tert- butanol had no effect on silymarin production. The exogenous addition of PA reversed the inhibitory action of nBuOH, both in control and MeJA-treated cultures. These results suggest that the enzyme PLD and its product PA mediate silymarin secretion to the medium of S. marianum cultures.
机译：黄素木聚糖水飞蓟素被释放到水飞蓟培养物的细胞外培养基中，其产生可以通过茉莉酸甲酯（MeJA）激发子来刺激。目前尚不清楚导致该响应的信号传导过程的顺序。据报道，在这项工作中，MeJA提高了磷脂酶D（PLD）酶的活性。使用Popa活性刺激剂帕斯汀（Mst）的治疗也增强了PLD，并导致水飞蓟素产量大幅增加。 PLD活性产物磷脂酸（PA）的应用促进了水飞蓟素的积累。通过在培养物中引入正丁醇（nBuOH）来改变PLD活性，它可抑制PLD产生的PA，可阻止MeJA或Mst诱导水飞蓟素的诱导，也可阻止其在非诱导培养物中的释放。用异丁醇，仲丁醇或叔丁醇处理对水飞蓟素的生产没有影响。在对照和MeJA处理的培养物中，PA的外源添加均可逆转nBuOH的抑制作用。这些结果表明，酶PLD及其产物PA介导水飞蓟素分泌到玛丽亚链霉菌培养物的培养基中。

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