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首页> 外文期刊>Journal of Experimental Botany >Trafficking of storage proteins in developing grain of wheat.
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Trafficking of storage proteins in developing grain of wheat.

机译：发育中的小麦籽粒中贮藏蛋白的运输。

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The processing properties of the wheat flour are largely determined by the structures and interactions of the grain storage proteins (also called gluten proteins) which form a continuous visco-elastic network in dough. Wheat gluten proteins are classically divided into two groups, the monomeric gliadins and the polymeric glutenins, with the latter being further classified into low molecular weight (LMW) and high molecular weight (HMW) subunits. The synthesis, folding and deposition of the gluten proteins take place within the endomembrane system of the plant cell. However, determination of the precise routes of trafficking and deposition of individual gluten proteins in developing wheat grain has been limited in the past by the difficulty of developing monospecific antibodies. To overcome this limitation, a single gluten protein (a LMW subunit) was expressed in transgenic wheat with a C-terminal epitope tag, allowing the protein to be located in the cells of the developing grain using highly specific antibodies. This approach was also combined with the use of wider specificity antibodies to compare the trafficking and deposition of different gluten protein groups within the same endosperm cells. These studies are in agreement with previous suggestions that two trafficking pathways occur in wheat, with the proteins either being transported via the Golgi apparatus into the vacuole or accumulating directly within the lumen of the ER. They also suggest that the same individual protein could be trafficked by either pathway, possibly depending on the stage of development, and that segregation of gluten proteins both between and within protein bodies may occur. (c) 2009 The Author(s).
机译：小麦粉的加工性能主要取决于谷物存储蛋白（也称为面筋蛋白）的结构和相互作用，这些蛋白在面团中形成连续的粘弹性网络。小麦面筋蛋白通常分为两类：单体麦醇溶蛋白和聚合物谷蛋白，后者进一步分为低分子量（LMW）和高分子量（HMW）亚基。面筋蛋白的合成，折叠和沉积发生在植物细胞的内膜系统内。但是，过去，由于开发单特异性抗体的困难，确定各个面筋蛋白在小麦籽粒中运输和沉积的精确途径的确定受到了限制。为了克服这一限制，在具有C端表位标签的转基因小麦中表达了单一的面筋蛋白（LMW亚基），从而可以使用高度特异性的抗体将其定位在发育中的谷物细胞中。该方法还与使用更广泛的特异性抗体相结合，以比较同一胚乳细胞内不同面筋蛋白基团的运输和沉积。这些研究与先前的建议一致，即小麦中存在两种运输途径，这些蛋白质要么通过高尔基体运输到液泡中，要么直接在ER内腔中蓄积。他们还暗示，可能通过发育途径，通过任何一种途径来贩运相同的蛋白质，并且可能在蛋白质体之间和内部发生面筋蛋白质的分离。 （c）2009作者。

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