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首页> 外文期刊>Journal of Experimental Botany >The cytoskeleton enhances gene expression in the response to the Harpin elicitor in grapevine.
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The cytoskeleton enhances gene expression in the response to the Harpin elicitor in grapevine.

机译：细胞骨架在葡萄中对Harpin激发子的应答中增强基因表达。

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摘要

The cytoskeleton undergoes dramatic reorganization during plant defence. This response is generally interpreted as part of the cellular repolarization establishing physical barriers against the invading pathogen. To gain insight into the functional significance of cytoskeletal responses for defence, two Vitis cell cultures that differ in their microtubular dynamics were used, and the cytoskeletal response to the elicitor Harpin in parallel to alkalinization of the medium as a fast response, and the activation of defence-related genes were followed. In one cell line derived from the grapevine cultivar 'Pinot Noir', microtubules contained mostly tyrosinylated alpha-tubulin, indicating high microtubular turnover, whereas in another cell line derived from the wild grapevine V. rupestris, the alpha-tubulin was strongly detyrosinated, indicating low microtubular turnover. The cortical microtubules were disrupted and actin filaments were bundled in both cell lines, but the responses were elevated in V. rupestris as compared with V. vinifera cv. 'Pinot Noir'. The cytoskeletal responsiveness correlated with elicitor-induced alkalinization and the expression of defence genes. Using resveratrol synthase and stilbene synthase as examples, it could be shown that pharmacological manipulation of microtubules could induce gene expression in the absence of elicitor. These findings are discussed with respect to a role for microtubules as positive regulators of defence-induced gene expression.
机译：在植物防御过程中，细胞骨架经历了巨大的重组。通常将此反应解释为细胞复极化的一部分，建立了针对入侵病原体的物理屏障。为了深入了解防御的细胞骨架反应的功能重要性，使用了两种微管动力学不同的Vitis细胞培养物，对激发子Harpin的细胞骨架反应与培养基的碱化（作为快速反应）和激活跟踪与国防相关的基因。在一个来自葡萄品种“黑比诺”的细胞系中，微管主要含有酪氨酸化的α-微管蛋白，表明微管的周转率很高，而在另一个来自野生葡萄V. rupestris的细胞系中，α-微管蛋白被强烈地脱酪氨酸化，表明微管周转率低。皮层微管被破坏，肌动蛋白丝被捆扎在两个细胞系中，但是与葡萄酿酒树相比，葡萄果树的反应提高了。 '黑比诺'。细胞骨架反应性与激发子诱导的碱化和防御基因的表达相关。以白藜芦醇合酶和二苯乙烯合酶为例，可以证明药理学操作的微管可以诱导基因表达，而没有引发剂。讨论了这些发现，涉及微管作为防御诱导基因表达的正向调节剂的作用。

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