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首页> 外文期刊>Journal of Experimental Botany >Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers.
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Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers.

机译：番茄花药中HsfA2和小的热激蛋白的发育和热应激调节表达。

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摘要

The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures.
机译：雄性生殖细胞对高温的高度敏感性可能是由于热应激反应不足所致。本文报道了在耐热番茄基因型发育中的花药中热应激系统的两个重要成员HsfA2和Hsp17-CII的综合表达分析结果。使用半定量和实时PCR进行了不同发育花药/花粉​​阶段的转录分析。使用原位RNA杂交对信使进行定位，并使用免疫印迹分析监测蛋白质积累。基于对基因和蛋白质表达谱的分析，HsfA2和Hsp17-CII在花药发育过程中受到良好调节，并在短期和长期热胁迫条件下进一步诱导。这些数据表明，HsfA2可能直接参与热胁迫期间番茄花药中保护机制的激活，从而可能有助于番茄在不利温度下结实。

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