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首页> 外文期刊>Journal of Experimental Botany >Two mitogen-activated protein kinase kinases, MKK1 and MEK2, are involved in wounding- and specialist lepidopteran herbivore Manduca sexta-induced responses in Nicotiana attenuata
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Two mitogen-activated protein kinase kinases, MKK1 and MEK2, are involved in wounding- and specialist lepidopteran herbivore Manduca sexta-induced responses in Nicotiana attenuata

机译：两种有丝分裂原激活的蛋白激酶激酶MKK1和MEK2参与了烟草变种和专性鳞翅目草食动物Manduca sexta诱导的烟草减毒反应

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摘要

In a wild tobacco plant, Nicotiana attenuata, two mitogen-activated protein kinases (MAPKs), salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), play central roles in modulating herbivory-induced phytohormone and anti-herbivore secondary metabolites. However, the identities of their upstream MAPK kinases (MAPKKs) were elusive. Ectopic overexpression studies in N. benthamiana and N. tabacum suggested that two MAPKKs, MKK1 and MEK2, may activate SIPK and WIPK. The homologues of MKK1 and MEK2 were cloned in N. attenuata (NaMKK1 and NaMEK2) and a virus-induced gene silencing approach was used to knock-down the transcript levels of these MAPKK genes. Plants silenced in NaMKK1 and NaMEK2 were treated with wounding or simulated herbivory by applying the oral secretions of the specialist herbivore Manduca sexta to wounds. MAPK activity assay indicated that after wounding or simulated herbivory NaMKK1 is not required for the phosphorylation of NaSIPK and NaWIPK; in contrast, NaMEK2 and other unknown MAPKKs are important for simulated herbivory-elicited activation of NaSIPK and NaWIPK, and after wounding NaMEK2 probably does not activate NaWIPK but plays a minor role in activating NaSIPK. Consistently, NaMEK2 and certain other MAPKKs, but not NaMKK1, are needed for wounding- and simulated herbivory-elicited accumulation of jasmonic acid (JA), JA-isoleucine, and ethylene. Furthermore, both NaMEK2 and NaMKK1 regulate the levels of trypsin proteinase inhibitors. The findings underscore the complexity of MAPK signalling pathways and highlight the importance of MAPKKs in regulating wounding- and herbivory-induced responses.
机译：在野生烟草植物中，两种有丝分裂原激活的蛋白激酶（MAPK），水杨酸诱导的蛋白激酶（SIPK）和伤口诱导的蛋白激酶（WIPK）在调节食草动物引起的植物激素和抗草食动物次生代谢产物。但是，其上游MAPK激酶（MAPKK）的身份难以捉摸。在本氏烟草和烟草中的异位过表达研究表明，两个MAPKKs MKK1和MEK2可能激活SIPK和WIPK。将MKK1和MEK2的同源物克隆到弱毒猪笼草（NaMKK1和NaMEK2）中，并使用病毒诱导的基因沉默方法来敲低这些MAPKK基因的转录水平。将NaMKK1和NaMEK2中沉默的植物通过在伤口上涂上专门的草食性曼杜卡（Medduca sexta）口腔分泌物进行伤口处理或模拟食草处理。 MAPK活性测定表明，受伤或模拟食草后，NaSIPK和NaWIPK的磷酸化不需要NaMKK1。相反，NaMEK2和其他未知的MAPKK对于模拟食草动物引起的NaSIPK和NaWIPK的激活很重要，并且在受伤后NaMEK2可能不会激活NaWIPK，但在激活NaSIPK方面起次要作用。一致地，需要用NaMEK2和某些其他MAPKK（而不是NaMKK1）来进行创伤和模拟的草食引起的茉莉酸（JA），JA-异亮氨酸和乙烯的积聚。此外，NaMEK2和NaMKK1均调节胰蛋白酶蛋白酶抑制剂的水平。这些发现强调了MAPK信号通路的复杂性，并强调了MAPKK在调节伤口和食草动物引起的反应中的重要性。

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