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首页> 外文期刊>Journal of Experimental Botany >Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase
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Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase

机译：脱落酸对大麦质体葡萄糖-6磷酸葡萄糖脱氢酶活性和表达的影响

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摘要

Total glucose-6-phosphate dehydrogenase (G6PDH) activity, protein abundance, and transcript levels of G6PDH isoforms were measured in response to exogenous abscisic acid (ABA) supply to barley (Hordeum vulgare cv Nure) hydroponic culture. Total G6PDH activity increased by 50% in roots treated for 12 h with exogenous 0.1 mM ABA. In roots, a considerable increase (35%) in plastidial P2-G6PDH transcript levels was observed during the first 3 h of ABA treatment. Similar protein variations were observed in immunoblotting analyses. In leaves, a 2-fold increase in total G6PDH activity was observed after ABA treatment, probably related to an increase in the mRNA level (increased by 50%) and amount of protein (increased by 85%) of P2-G6PDH. Together these results suggest that the plastidial P2-isoform plays an important role in ABA-treated barley plants.
机译：响应于向大麦（Hordeum vulgare cv Nure）水培培养物中供应的外源脱落酸（ABA），测量了总葡萄糖-6-磷酸脱氢酶（G6PDH）活性，蛋白质丰度和G6PDH亚型的转录水平。在用外源0.1 mM ABA处理12 h的根中，总G6PDH活性增加了50％。在根部，在ABA处理的前3小时内观察到质体P2-G6PDH转录水平显着增加（35％）。在免疫印迹分析中观察到相似的蛋白质变异。在叶片中，ABA处理后的总G6PDH活性增加了2倍，这可能与P2-G6PDH的mRNA水平增加（增加了50％）和蛋白质含量（增加了85％）有关。这些结果共同表明，质体P2-同工型在ABA处理的大麦植物中起重要作用。

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