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首页> 外文期刊>Journal of Experimental Botany >Functional diversity inside the Arabidopsis polyamine oxidase gene family
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Functional diversity inside the Arabidopsis polyamine oxidase gene family

机译：拟南芥多胺氧化酶基因家族内部的功能多样性

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Polyamine oxidases (PAOs) are FAD-dependent enzymes involved in polyamine catabolism. All so far characterized PAOs from monocotyledonous plants, such as the apoplastic maize PAO, oxidize spermine (Spm) and spermidine (Spd) to produce 1,3-diaminopropane, H2O2, and an aminoaldehyde, and are thus considered to be involved in a terminal catabolic pathway. Mammalian PAOs oxidize Spm or Spd (and/or their acetyl derivatives) differently from monocotyledonous PAOs, producing Spd or putrescine, respectively, in addition to H2O2 and an aminoaldehyde, and are therefore involved in a polyamine back-conversion pathway. In Arabidopsis thaliana, five PAOs (AtPAO1-AtPAO5) are present with cytosolic or peroxisomal localization and three of them (the peroxisomal AtPAO2, AtPAO3, and AtPAO4) form a distinct PAO subfamily. Here, a comparative study of the catalytic properties of recombinant AtPAO1, AtPAO2, AtPAO3, and AtPAO4 is presented, which shows that all four enzymes strongly resemble their mammalian counterparts, being able to oxidize the common polyamines Spd and/or Spm through a polyamine back-conversion pathway. The existence of this pathway in Arabidopsis plants is also evidenced in vivo. These enzymes are also able to oxidize the naturally occurring uncommon polyamines norspermine and thermospermine, the latter being involved in important plant developmental processes. Furthermore, data herein reveal some important differences in substrate specificity among the various AtPAOs, which suggest functional diversity inside the AtPAO gene family. These results represent a new starting point for further understanding of the physiological role(s) of the polyamine catabolic pathways in plants.
机译：多胺氧化酶（PAO）是FAD依赖的酶，参与多胺分解代谢。迄今为止，来自单子叶植物的特征化PAO（例如，外生玉米PAO）氧化精胺（Spm）和亚精胺（Spd）以产生1,3-二氨基丙烷，H2O2和氨基醛，因此被认为与末端有关分解代谢途径。哺乳动物的PAOs与单子叶的PAOs氧化Spm或Spd（和/或它们的乙酰基衍生物）的方式不同，除H2O2和氨基醛外，它们分别产生Spd或腐胺，因此参与了多胺的反向转化途径。在拟南芥中，存在五个具有胞质或过氧化物酶体定位的PAO（AtPAO1-AtPAO5），其中三个（过氧化物酶体AtPAO2，AtPAO3和AtPAO4）形成一个不同的PAO亚家族。在这里，对重组AtPAO1，AtPAO2，AtPAO3和AtPAO4的催化性能进行了比较研究，结果表明所有四种酶都非常类似于它们的哺乳动物对应物，能够通过多胺氧化普通多胺Spd和/或Spm。 -转化途径。体内还证实了该途径在拟南芥植物中的存在。这些酶还能够氧化天然不常见的多胺鸟精胺和热精胺，后者参与重要的植物发育过程。此外，本文的数据揭示了各种AtPAO之间底物特异性的一些重要差异，这表明AtPAO基因家族内部的功能多样性。这些结果代表了进一步了解植物中多胺分解代谢途径的生理作用的新起点。

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