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首页> 外文期刊>Journal of Experimental Botany >Expression profiles of differentially regulated genes during the early stages of apple flower infection with Erwinia amylovora.
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Expression profiles of differentially regulated genes during the early stages of apple flower infection with Erwinia amylovora.

机译：苹果花欧文氏杆菌感染苹果花早期的差异调节基因表达谱。

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摘要

To identify genes involved in the response to the fire blight pathogen Erwinia amylovora in apple (Malus x domestica), expression profiles were investigated using an apple oligo (70-mer) array representing 40, 000 genes. Blossoms of a fire blight-susceptible apple cultivar Gala were collected from trees growing in the orchard, placed on a tray in the laboratory, and spray-inoculated with a suspension of E. amylovora at a concentration of 108 cfu ml-1. Uninoculated detached flowers served as controls at each time point. Expression profiles were captured at three different time points post-inoculation at 2, 8, and 24 h, together with those at 0 h (uninoculated). A total of about 3500 genes were found to be significantly modulated in response to at least one of the three time points. Among those, a total of 770, 855, and 1002 genes were up-regulated, by 2-fold, at 2, 8, and 24 h following inoculation, respectively; while, 748, 1024, and 1455 genes were down-regulated, by 2-fold, at 2, 8, and 24 h following inoculation, respectively. Over the three time points post-inoculation, 365 genes were commonly up-regulated and 374 genes were commonly down-regulated. Both sets of genes were classified based on their functional categories. The majority of up-regulated genes were involved in metabolism, signal transduction, signalling, transport, and stress response. A number of transcripts encoding proteins/enzymes known to be up-regulated under particular biotic and abiotic stress were also up-regulated following E. amylovora treatment. Those up- or down-regulated genes encode transcription factors, signaling components, defense-related, transporter, and metabolism, all of which have been associated with disease responses in Arabidopsis and rice, suggesting similar response pathways are involved in apple blossoms.Digital Object Identifier http://dx.doi.org/10.1093/jxb/err147
机译：为了鉴定苹果对火疫病原体 Aylovia amylovora 的响应中涉及的基因，使用苹果研究了表达谱寡核苷酸（70-mer）阵列，代表40，000个基因。从在果园中生长的树木上收集易受火疫病侵害的苹果栽培品种Gala的花朵，将其放在实验室的托盘上，并用e悬浮液进行喷雾接种。支链淀粉的浓度为10 8 cfu ml -1 。未接种的离体花在每个时间点均作为对照。在接种后2、8和24小时的三个不同时间点以及在0小时（未接种）的时间分别捕获表达谱。发现总共响应约三个时间点中的至少一个，显着调节了约3500个基因。其中，分别在接种后2、8和24小时将总共770、855和1002个基因上调了2倍。而748、1024和1455个基因分别在接种后2、8和24小时下调了2倍。在接种后的三个时间点，365个基因通常被上调，而374个基因通常被下调。两组基因均根据其功能类别进行分类。大多数上调的基因参与代谢，信号转导，信号传导，转运和应激反应。 E后，许多编码在特定生物和非生物胁迫下被上调的编码蛋白质/酶的转录物也被上调。支链淀粉的治疗。这些上调或下调的基因编码转录因子，信号传导成分，防御相关，转运蛋白和代谢，所有这些都与拟南芥和水稻的疾病反应有关，这表明类似的反应途径是涉及苹果开花。数字对象标识符http://dx.doi.org/10.1093/jxb/err147

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