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首页> 外文期刊>Journal of Experimental Botany >The barley amo1 locus is tightly linked to the starch synthase IIIa gene and negatively regulates expression of granule-bound starch synthetic genes.
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The barley amo1 locus is tightly linked to the starch synthase IIIa gene and negatively regulates expression of granule-bound starch synthetic genes.

机译：大麦 amo 1位点与淀粉合酶 IIIa 基因紧密相连，并负面调节颗粒结合的淀粉合成基因的表达。

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In this study of barley starch synthesis, the interaction between mutations at the sex6 locus and the amo1 locus has been characterized. Four barley genotypes, the wild type, sex6, amo1, and the amo1sex6 double mutant, were generated by backcrossing the sex6 mutation present in Himalaya292 into the amo1 "high amylose Glacier". The wild type, amo1, and sex6 genotypes gave starch phenotypes consistent with previous studies. However, the amo1sex6 double mutant yielded an unexpected phenotype, a significant increase in starch content relative to the sex6 phenotype. Amylose content (as a percentage of starch) was not increased above the level observed for the sex6 mutation alone; however, on a per seed basis, grain from lines containing the amo1 mutation (amo1 mutants and amo1sex6 double mutants) synthesize significantly more amylose than the wild-type lines and sex6 mutants. The level of granule-bound starch synthase I (GBSSI) protein in starch granules is increased in lines containing the amo1 mutation (amo1 and amo1sex6). In the amo1 genotype, starch synthase I (SSI), SSIIa, starch branching enzyme IIa (SBEIIa), and SBEIIb also markedly increased in the starch granules. Genetic mapping studies indicate that the ssIIIa gene is tightly linked to the amo1 locus, and the SSIIIa protein from the amo1 mutant has a leucine to arginine residue substitution in a conserved domain. Zymogram analysis indicates that the amo1 phenotype is not a consequence of total loss of enzymatic activity although it remains possible that the amo1 phenotype is underpinned by a more subtle change. It is therefore proposed that amo1 may be a negative regulator of other genes of starch synthesis
机译：在这项对大麦淀粉合成的研究中，已表征了 sex 6位点和 amo 1位点的突变之间的相互作用。四种大麦基因型，野生型性 6，a mo 1和 amo 1 性 6双重突变体是通过将喜马拉雅山292中存在的 sex 6突变回交到 amo 1“高直链淀粉冰川”中而产生的。野生型 amo 1和性别 6基因型产生的淀粉表型与以前的研究一致。但是， amo 1 sex 6双突变体产生了意想不到的表型，相对于 sex 6表型，淀粉含量显着增加。直链淀粉含量（以淀粉的百分比计）没有增加到超过单独的 s 6突变所观察到的水平;但是，基于每个种子，谷物中包含 amo 1突变（ amo 1突变体和 amo 1 性 6个双突变体）合成的直链淀粉比野生型和 sex 6个突变体多得多。在包含 amo 1突变（ amo 1和 amo 1 性别 6）。在 amo 基因型中，淀粉颗粒中的淀粉合酶I（SSI），SSIIa，淀粉分支酶IIa（SBEIIa）和SBEIIb也显着增加。遗传图谱研究表明 ssIIIa 基因与 amo 1位点紧密相连，并且 amo 1突变体的SSIIIa蛋白具有亮氨酸保守域中精氨酸残基取代。字形分析表明， amo 1表型不是酶活性完全丧失的结果，尽管 amo 1表型仍然有可能被更微妙的变化所支撑。因此建议 amo 1可能是淀粉合成其他基因的负调控因子

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