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首页> 外文期刊>Journal of Experimental Botany >MicroRNA activity in the Arabidopsis male germline
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MicroRNA activity in the Arabidopsis male germline

机译：拟南芥雄性种系中的MicroRNA活性

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摘要

Most of the core proteins involved in the microRNA (miRNA) pathway in plants have been identified, and almost simultaneously hundreds of miRNA sequences processed in the Arabidopsis sporophyte have been discovered by exploiting next-generation sequencing technologies. However, there is very limited understanding about potentially distinct mechanisms of post-transcriptional regulation between different cell lineages. In this review the focus is on the Arabidopsis male gametophyte (pollen), where the germline differentiates after meiosis giving rise to the male gametes. Based on comparative analysis of miRNAs identified in sperm cells by in-depth sequencing, their possible functions during germ cell specification and beyond fertilization are discussed. In addition, 25 potentially novel miRNAs processed in sperm cells and pollen were identified, as well as enriched variations in the sequence length of known miRNAs, which might indicate subfunctionalization by association with a putative germline-specific Argonaute complex. ARGONAUTE 5 (AGO5), by close homology to AGO1 and localizing preferentially to the sperm cell cytoplasm in mature pollen, may be part of such a complex.
机译：已鉴定出植物中涉及microRNA（miRNA）途径的大多数核心蛋白，并且利用下一代测序技术几乎同时发现了拟南芥孢子体中加工的数百个miRNA序列。但是，对不同细胞谱系之间转录后调控的潜在独特机制的了解非常有限。在这篇综述中，重点是拟南芥雄配子体（花粉），其中减数分裂产生雄配子后，种系分化。在对精子细胞中通过深度测序鉴定出的miRNA进行比较分析的基础上，讨论了它们在生殖细胞鉴定期间以及受精后的可能功能。此外，鉴定了在精子细胞和花粉中加工的25种潜在的新颖miRNA，以及已知miRNA序列长度的丰富变化，这可能表明与假定的种系特异性Argonaute复合物结合而亚功能化。通过与AGO1高度同源并优先定位在成熟花粉中的精子细胞质中的ARGONAUTE 5（AGO5）可能是这种复合物的一部分。

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