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首页> 外文期刊>Journal of Experimental Botany >Effects of molybdenum deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana
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Effects of molybdenum deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana

机译：钼缺乏和钼酸盐转运蛋白MOT1缺陷对拟南芥转录本积累和氮/硫代谢的影响

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摘要

Molybdenum (Mo) is a micronutrient essential for plant growth, as several key enzymes of plant metabolic pathways contain Mo cofactor in their catalytic centres. Mo-containing oxidoreductases include nitrate reductase, sulphite oxidase, xanthine dehydrogenase, and aldehyde oxidase. These are involved in nitrate assimilation, sulphite detoxification, purine metabolism or the synthesis of abscisic acid, auxin and glucosinolates in plants. To understand the effects of Mo deficiency and a mutation in a molybdate transporter, MOT1, on nitrogen and sulphur metabolism in Arabidopsis thaliana, transcript and metabolite profiling of the mutant lacking MOT1 was conducted in the presence or absence of Mo. Transcriptome analysis revealed that Mo deficiency had impacts on genes involved in metabolisms, transport, stress responses, and signal transductions. The transcript level of a nitrate reductase NR1 was highly induced under Mo deficiency in mot1-1. The metabolite profiles were analysed further by using gas chromatography time-of-flight mass spectrometry, capillary electrophoresis time-of-flight mass spectrometry, and ultra high performance liquid chromatography. The levels of amino acids, sugars, organic acids, and purine metabolites were altered significantly in the Mo-deficient plants. These results are the first investigation of the global effect of Mo nutrition and MOT1 on plant gene expressions and metabolism.
机译：钼（Mo）是植物生长必不可少的微量营养素，因为植物代谢途径中的几种关键酶在其催化中心均含有Mo辅因子。含钼的氧化还原酶包括硝酸盐还原酶，亚硫酸盐氧化酶，黄嘌呤脱氢酶和醛氧化酶。这些涉及植物中的硝酸盐吸收，亚硫酸盐解毒，嘌呤代谢或脱落酸，生长素和芥子油苷的合成。为了了解钼缺乏和钼酸盐转运蛋白MOT1的突变对拟南芥中氮和硫代谢的影响，在有或没有Mo的情况下，对缺乏MOT1的突变体进行转录和代谢产物分析。转录组分析显示，Mo缺乏对涉及代谢，转运，应激反应和信号转导的基因有影响。在mot1-1的Mo缺乏下，高度诱导了硝酸还原酶NR1的转录水平。通过使用气相色谱飞行时间质谱，毛细管电泳飞行时间质谱和超高效液相色谱进一步分析代谢物谱。缺钼植物中氨基酸，糖，有机酸和嘌呤代谢产物的水平发生了显着变化。这些结果是对Mo营养和MOT1对植物基因表达和代谢的全球影响的首次调查。

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