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首页> 外文期刊>Journal of Experimental Botany >Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.
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Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.

机译：丝氨酸乙酰转移酶的过表达在谷物豆类的发育种子中产生大量的O-乙酰丝氨酸和游离半胱氨酸增加。

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摘要

There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosyntheis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein.
机译：已经进行了许多尝试，通过改变转基因植物叶片中的生物合成途径来增加营养必需的硫氨基酸的浓度。该报告描述了发育中种子中半胱氨酸生物合成的首次修饰;那些豆科植物，羽扇豆羽扇豆（Lupinus angustifolius，L。）。来自拟南芥的丝氨酸乙酰基转移酶（SAT）（AtSAT1或AtSerat 2; 1）在发育中的羽扇豆胚胎中的表达与SAT的直接产物O-乙酰丝氨酸（OAS）的浓度增加多达5倍相关，游离半胱氨酸含量高达26倍，导致这些代谢产物的体内最高浓度出现了一些报道。尽管SAT过表达的发育中胚胎中的游离半胱氨酸发生了巨大变化，但发育中的胚胎中游离蛋氨酸的浓度以及成熟种子中总半胱氨酸和蛋氨酸的浓度没有明显变化。分离的SAT转基因和编码富含蛋氨酸和半胱氨酸的葵花籽贮藏蛋白的转基因的F种子在发育过程中也具有增加的OAS和游离半胱氨酸，但没有游离蛋氨酸，并且成熟种子的总硫氨基酸没有增加与缺乏SAT过表达的对照相比。数据支持这样的观点，即半胱氨酸生物合成途径在发育中的种子中起作用，并表明SAT活性限制了半胱氨酸生物合成，但是在硫磺充足的条件下，半胱氨酸的供应并不限制蛋氨酸的生物合成或成熟羽扇豆胚中的贮藏蛋白合成。 。 OAS和游离蛋氨酸而不是游离半胱氨酸被认为是控制富含半胱氨酸的种子贮藏蛋白基因表达的信号代谢产物。

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