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首页> 外文期刊>Journal of Experimental Botany >A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis).
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A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis).

机译：bHLH转录因子 DvIVS 参与大丽花（ Dahlia variabilis ）中花色苷合成的调控。

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摘要

Dahlias (Dahlia variabilis) exhibit a wide range of flower colours because of accumulation of anthocyanin and other flavonoids in their ray florets. Two lateral mutants were used that spontaneously occurred in 'Michael J' (MJW) which has yellow ray florets with orange variegation. MJOr, a bud mutant producing completely orange ray florets, accumulates anthocyanins, flavones, and butein, and MJY, another mutant producing completely yellow ray florets, accumulates flavones and butein. Reverse transcription-PCR analysis showed that expression of chalcone synthase 1 (DvCHS1), flavanone 3-hydroxylase (DvF3H), dihydroflavonol 4-reductase (DvDFR), anthocyanidin synthase (DvANS), and DvIVS encoding a basic helix-loop-helix transcription factor were suppressed, whereas that of chalcone isomerase (DvCHI) and DvCHS2, another CHS with 69% nucleotide identity with DvCHS1, was not suppressed in the yellow ray florets of MJY. A 5.4 kb CACTA superfamily transposable element, transposable element of Dahlia variabilis 1 (Tdv1), was found in the fourth intron of the DvIVS gene of MJW and MJY, and footprints of Tdv1 were detected in the variegated flowers of MJW. It is shown that only one type of DvIVS gene was expressed in MJOr, whereas these plants are likely to have three types of the DvIVS gene. On the basis of these results, the mechanism regulating the formation of orange and yellow ray florets in dahlia is discussed.
机译：大丽花（ Dahlia variabilis ）由于花色苷和其他类黄酮在其射线小花中积累，因此具有多种花色。使用了两个侧向突变体，它们自发出现在“ Michael J”（MJW）中，该突变体具有黄色射线的小花和橙色的杂色。 MJOr是产生完全橙色射线小花的芽突变体，累积花青素，黄酮和丁烯，而MJY是产生完全黄色射线小花的另一突变体，累积黄酮和丁烯。逆转录-PCR分析显示查尔酮合酶1 （ DvCHS1 ），黄烷酮3-羟化酶（ DvF3H ），二氢黄酮醇4-还原酶（ DvDFR ），花色素苷合酶（ DvANS ）和 DvIVS < / i>抑制了基本的螺旋-环-螺旋转录因子的编码，而查耳酮异构酶（ DvCHI ）和 DvCHS2 的编码则被抑制了。与 DvCHS1 具有69％核苷酸同一性的i> CHS 在MJY的黄色射线小花中没有被抑制。在 DvIVS 基因的第四个内含子中发现了一个5.4 kb CACTA超家族转座因子，即大丽花变种1 （ Tdv1 ）的转座因子。在MJW的杂色花中检测到MJW和MJY，以及 Tdv1 的足迹。结果表明，在MJOr中仅表达一种类型的 DvIVS 基因，而这些植物很可能具有三种类型的 DvIVS 基因。根据这些结果，讨论了调节大丽花中橙色和黄色射线小花形成的机理。

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