...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Modulation of plant TPC channels by polyunsaturated fatty acids.
【24h】

Modulation of plant TPC channels by polyunsaturated fatty acids.

机译：多不饱和脂肪酸对植物TPC通道的调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana mesophyll cells and arachidonic acid (AA) was chosen as a model molecule for PUFAs. Our study was extended to different PUFAs including the endogenous alpha-linolenic acid (ALA). The addition of micromolar concentrations of AA reversibly inhibited the SV channel decreasing the maximum open probability and shifting the half activation voltage to positive values. Comparing the effects of different PUFAs, it was found that the length of the lipophilic acyl chain, the number of double bonds and the polar head were critical for channel modulation. The experimental data can be reproduced by a simple three-state model, in which PUFAs do not interact directly with the voltage sensors but affect the voltage-independent transition that leads the channel from the open state to the closed configuration. The results indicate that lipids play an important role in co-ordinating ion channel activities similar to what is known from animal cells.
机译：多不饱和脂肪酸（PUFA）是几种动物离子通道的强大调节剂。此处显示了PUFA强烈影响植物TPC1基因编码的慢泡（SV）通道的活性。膜片钳技术应用于从胡萝卜主根和拟南芥叶肉细胞分离的液泡，并选择花生四烯酸（AA）作为PUFAs的模型分子。我们的研究扩展到包括内源性α-亚麻酸（ALA）在内的不同PUFA。加入微摩尔浓度的AA可逆地抑制了SV通道，从而降低了最大打开概率并将半激活电压移至正值。比较不同PUFA的影响，发现亲脂酰基链的长度，双键数和极性头对于通道调制至关重要。实验数据可以通过简单的三态模型重现，其中PUFA不会直接与电压传感器相互作用，而是会影响电压无关的过渡，从而将通道从打开状态变为关闭状态。结果表明，脂质在协调离子通道活性方面起着重要作用，类似于从动物细胞中已知的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号