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首页> 外文期刊>Journal of Experimental Botany >Response of mitochondrial thioredoxin PsTrxo1, antioxidant enzymes, and respiration to salinity in pea (Pisum sativum L.) leaves
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Response of mitochondrial thioredoxin PsTrxo1, antioxidant enzymes, and respiration to salinity in pea (Pisum sativum L.) leaves

机译：豌豆（Pisum sativum L.）叶片线粒体硫氧还蛋白PsTrxo1，抗氧化酶和呼吸对盐度的响应

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Mitochondria play an essential role in reactive oxygen species (ROS) signal transduction in plants. Redox regulation is an essential feature of mitochondrial function, with thioredoxin (Trx), involved in disulphide/dithiol interchange, playing a prominent role. To explore the participation of mitochondrial PsTrxo1, Mn-superoxide dismutase (Mn-SOD), peroxiredoxin (PsPrxII F), and alternative oxidase (AOX) under salt stress, their transcriptional and protein levels were analysed in pea plants growing under 150 mM NaCl for a short and a long period. The activities of mitochondrial Mn-SOD and Trx together with the in vivo activities of the alternative pathway (AP) and the cytochrome pathway (CP) were also determined, combined with the characterization of the plant physiological status as well as the mitochondrial oxidative indicators. The analysis of protein and mRNA levels and activities revealed the importance of the post-transcriptional and post-translational regulation of these proteins in the response to salt stress. Increases in AOX protein amount correlated with increases in AP capacity, whereas in vivo AP activity was maintained under salt stress. Similarly, Mn-SOD activity was also maintained. Under all the stress treatments, photosynthesis, stomatal conductance, and CP activity were decreased although the oxidative stress in leaves was only moderate. However, an increase in lipid peroxidation and protein oxidation was found in mitochondria isolated from leaves under the short-term salinity conditions. In addition, an increase in mitochondrial Trx activity was produced in response to the long-term NaCl treatment. The results support a role for PsTrxo1 as a component of the defence system induced by NaCl in pea mitochondria, providing the cell with a mechanism by which it can respond to changing environment protecting mitochondria from oxidative stress together with Mn-SOD, AOX, and PrxII F.
机译：线粒体在植物中的活性氧（ROS）信号转导中起重要作用。氧化还原调节是线粒体功能的基本特征，硫氧还蛋白（Trx）参与二硫键/二硫醇的交换，起着重要的作用。为了研究盐胁迫下线粒体PsTrxo1，Mn超氧化物歧化酶（Mn-SOD），过氧化物酶（PsPrxII F）和替代氧化酶（AOX）的参与，分析了在150 mM NaCl下生长的豌豆植物中它们的转录和蛋白质水平。短期和长期。还确定了线粒体Mn-SOD和Trx的活性，以及​​替代途径（AP）和细胞色素途径（CP）的体内活性，并结合了植物生理状态的表征以及线粒体的氧化指标。蛋白质和mRNA水平及活性的分析揭示了这些蛋白质在对盐胁迫的响应中转录后和翻译后调节的重要性。 AOX蛋白量的增加与AP容量的增加相关，而体内AP活性在盐胁迫下得以维持。同样，也保持了Mn-SOD活性。在所有胁迫处理下，尽管叶片中的氧化胁迫仅为中等水平，但光合作用，气孔导度和CP活性均降低。然而，在短期盐度条件下，从叶中分离的线粒体中发现脂质过氧化和蛋白质氧化增加。另外，响应于长期的NaCl处理，线粒体Trx活性增加。这些结果支持PsTrxo1作为豌豆线粒体中NaCl诱导的防御系统的组成部分，为细胞提供了一种机制，通过该机制它可以响应不断变化的环境保护线粒体免受Mn-SOD，AOX和PrxII的氧化胁迫F。

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