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首页> 外文期刊>Journal of Experimental Botany >Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis.
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Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis.

机译：拟南芥中编码电压依赖性阴离子通道的基因家族的分子和遗传特征。

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摘要

The voltage-dependent anion channel (VDAC), a major outer mitochondrial membrane protein, is thought to play an important role in energy production and apoptotic cell death in mammalian systems. However, the function of VDACs in plants is largely unknown. In order to determine the individual function of plant VDACs, molecular and genetic analysis was performed on four VDAC genes, VDAC1-VDAC4, found in Arabidopsis thaliana. VDAC1 and VDAC3 possess the eukaryotic mitochondrial porin signature (MPS) in their C-termini, while VDAC2 and VDAC4 do not. Localization analysis of VDAC-green fluorescent protein (GFP) fusions and their chimeric or mutated derivatives revealed that the MPS sequence is important for mitochondrial localization. Through the functional analysis of vdac knockout mutants due to T-DNA insertion, VDAC2 and VDAC4 which are expressed in the whole plant body are important for various physiological functions such as leaf development, the steady state of the mitochondrial membrane potential, and pollen development. Moreover, it was demonstrated that VDAC1 is not only necessary for normal growth but also important for disease resistance through regulation of hydrogen peroxide generation.
机译：电压依赖性阴离子通道（VDAC）是主要的线粒体外膜蛋白，被认为在哺乳动物系统的能量产生和凋亡细胞死亡中起着重要作用。但是，植物中VDAC的功能很大程度上未知。为了确定植物VDAC的个别功能，对拟南芥中发现的四个 VDAC 基因 VDAC1-VDAC4 进行了分子和遗传分析。 >。 VDAC1和VDAC3在其C末端具有真核线粒体孔蛋白签名（MPS），而VDAC2和VDAC4没有。 VDAC绿色荧光蛋白（GFP）融合蛋白及其嵌合或突变的衍生物的定位分析表明，MPS序列对于线粒体定位很重要。通过对由于插入T-DNA而导致的 acd 敲除突变体的功能进行分析，在整个植物体内表达的VDAC2和VDAC4对于各种生理功能（例如叶片发育，线粒体的稳态）都很重要。膜电位和花粉发育。此外，已证明VDAC1不仅对于正常生长是必需的，而且通过调节过氧化氢的产生对于疾病抗性也很重要。

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