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首页> 外文期刊>Journal of Experimental Botany >The role for the exocyst complex subunits Exo70B2 and Exo70H1 in the plant-pathogen interaction
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The role for the exocyst complex subunits Exo70B2 and Exo70H1 in the plant-pathogen interaction

机译：外囊复合物亚基Exo70B2和Exo70H1在植物-病原体相互作用中的作用

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Recently, the octameric vesicle-tethering complex exocyst was found in plants and its importance for Arabidopsis morphogenesis was demonstrated. Exo70 exocyst subunits in plants, unlike in yeasts and mammals, are represented by a multigene family, comprising 23 members in Arabidopsis. For Exo70B2 and Exo70H1 paralogues, transcriptional up-regulation was confirmed on treatment with an elicitor peptide, elf18, derived from the bacterial elongation factor. Their ability to participate in the exocyst complex formation was inferred by the interaction of both the Exo70s with several other exocyst subunits using the yeast two-hybrid system. Arabidopsis plants mutated in these two genes were used to analyse their local reaction upon inoculation with Pseudomonas syringae pv. maculicola and the fungal pathogen Blumeria graminis f. sp. hordei. The Pseudomonas sensitivity test revealed enhanced susceptibility for the two exo70B2 and one H1 mutant lines. After Blumeria inoculation, an increase in the proportion of abnormal papilla formation, with an unusual wide halo made of vesicle-like structures, was found in exo70B2 mutants. Intracellular localization of both Exo70 proteins was studied following a GFP fusion assay and Agrobacterium-mediated transient expression of the constructs in Nicotiana benthamiana leaf epidermis. GFP-Exo70H1 localizes in the vesicle-like structures, while GFP-Exo70B2 is localized mainly in the cytoplasm. It is concluded that both Exo70B2 and Exo70H1 are involved in the response to pathogens, with Exo70B2 having a more important role in cell wall apposition formation related to plant defence.
机译：最近，在植物中发现了八聚体水泡-束缚复合物外囊，并且证明了其对拟南芥形态发生的重要性。与酵母和哺乳动物不同，植物中的Exo70外囊亚基以多基因家族为代表，该家族在拟南芥中包含23个成员。对于Exo70B2和Exo70H1旁系同源物，在用源自细菌延伸因子的激发子肽elf18进行处理后，确认转录上调。通过使用酵母双杂交系统，两个Exo70与两个其他囊外亚基的相互作用，推断它们参与囊外复合物形成的能力。在这两个基因中突变的拟南芥植物用于接种丁香假单胞菌PV后分析其局部反应。 maculicola和真菌病原体Blumeria graminis f。 sp。部落假单胞菌敏感性测试表明，对两个exo70B2和一个H1突变株的敏感性增强。接种Blumeria后，在exo70B2突变体中发现了异常的乳头形成比例增加，具有由小泡样结构构成的异常宽的光环。在GFP融合测定法和农杆菌介导的烟草本生叶表皮中该构建体的瞬时表达之后，研究了两种Exo70蛋白的细胞内定位。 GFP-Exo70H1位于小泡状结构中，而GFP-Exo70B2主要位于细胞质中。结论是，Exo70B2和Exo70H1都参与了对病原体的反应，其中Exo70B2在与植物防御相关的细胞壁并置形成中具有更重要的作用。

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