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首页> 外文期刊>Journal of Experimental Botany >Proteomic analysis by two-dimensional differential in gel electrophoresis (2D DIGE) of the early response of Pisum sativum to Orobanche crenata
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Proteomic analysis by two-dimensional differential in gel electrophoresis (2D DIGE) of the early response of Pisum sativum to Orobanche crenata

机译：蛋白质组学分析的二维电泳凝胶电泳（2D DIGE）中的Pisum sativum对Orobanche crenata的早期反应

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摘要

Crenate broomrape (Orobanche crenata) is considered to be the major constraint for legume crops in Mediterranean countries. Strategies of control have been developed, but only marginal successes have been achieved. For the efficient control of the parasite, a better understanding of its interaction and associated resistance mechanisms at the molecular level is required. The pea response to this parasitic plant and the molecular basis of the resistance was studied using a proteomic approach based on 2D DIGE and MALDI-MSMS analysis. For this purpose, two genotypes showing different levels of resistance to O. crenata, as well as three time points (21, 25, and 30 d after inoculation) have been compared. Multivariate statistical analysis identified 43 differential protein spots under the experimental conditions (genotypes/treatments), 22 of which were identified using a combination of peptide mass fingerprinting (PMF) and MSMS fragmentation. Most of the proteins identified were metabolic and stress-related proteins and a high percentage of them (86%) matched with specific proteins of legume species. The behaviour pattern of the identified proteins suggests the existence of defence mechanisms operating during the early stages of infection that differed in both genotypes. Among these, several proteins were identified with protease activity which could play an important role in preventing the penetration and connection to the vascular system of the parasite. Our data are discussed and compared with those previously obtained in pea and Medicago truncatula.
机译：新月形countries帚（Orobanche crenata）被认为是地中海国家豆类作物的主要限制因素。已经制定了控制策略，但仅取得了很小的成功。为了有效控制寄生虫，需要在分子水平上更好地了解其相互作用和相关的耐药机制。使用基于2D DIGE和MALDI-MSMS分析的蛋白质组学方法研究了豌豆对这种寄生植物的反应和抗性的分子基础。为了这个目的，已经比较了显示出对蜡菊的抗性水平不同的两个基因型以及三个时间点（接种后21、25和30 d）。多变量统计分析在实验条件（基因型/治疗）下鉴定出43个差异蛋白斑点，其中22个是通过使用肽质量指纹图谱（PMF）和MSMS片段化技术组合鉴定的。鉴定出的大多数蛋白质是与代谢和应激相关的蛋白质，其中很大一部分（86％）与豆类物种的特定蛋白质相匹配。鉴定出的蛋白质的行为模式表明存在在两种基因型不同的感染早期阶段起作用的防御机制。其中，鉴定出具有蛋白酶活性的几种蛋白质，这些蛋白质可在防止寄生虫的渗透和与血管系统的连接中起重要作用。我们的数据进行了讨论，并与先前在豌豆和苜蓿中得到的数据进行了比较。

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