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首页> 外文期刊>Journal of Experimental Botany >The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination.
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The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination.

机译：质体外被膜蛋白OEP16在ABA控制的种子发育和发芽过程中影响代谢通量。

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摘要

Previously, the OEP16.1 channel pore in the outer envelope membrane of mature pea (Pisum sativum) chloroplasts in vitro has been characterized to be selective for amino acids. Isolation of OEP16.2, a second OEP16 isoform from pea, in the current study allowed membrane localization and gene expression of OEP16 to be followed throughout seed development and germination of Arabidopsis thaliana and P. sativum. Thereby it can be shown on the transcript and protein level that the isoforms OEP16.1 and OEP16.2 in both plant species are alternating: whereas OEP16.1 is prominent in early embryo development and first leaves of the growing plantlet, OEP16.2 dominates in late seed development stages, which are associated with dormancy and desiccation, as well as early germination events. Further, OEP16.2 expression in seeds is under control of the phytohormone abscisic acid (ABA), leading to an ABA-hypersensitive phenotype of germinating oep16 knockout mutants. In consequence, the loss of OEP16 causes metabolic imbalance, in particular that of amino acids during seed development and early germination. It is thus concluded that in vivo OEP16 most probably functions in shuttling amino acids across the outer envelope of seed plastids.
机译：以前，成熟豌豆（Pisum sativum ）叶绿体的外壳膜中的OEP16.1通道孔已被表征为对氨基酸具有选择性。在目前的研究中，从豌豆中分离出第二种OEP16亚型OEP16.2，可以在整个拟南芥和拟南芥种子发芽和发芽过程中跟踪OEP16的膜定位和基因表达。仙人掌。因此，从转录本和蛋白质水平可以看出，两种植物中的同工型OEP16.1和OEP16.2是交替的：而OEP16.1在早期胚胎发育和正在生长的幼苗的初叶中突出，而OEP16.2占主导地位在种子发育后期，与休眠，干燥以及早期发芽事件有关。此外，种子中的OEP16.2表达受植物激素脱落酸（ABA）的控制，导致发芽 oep16 敲除突变体的ABA超敏表型。因此，OEP16的丢失会引起代谢失衡，特别是在种子发育和早期发芽过程中氨基酸失衡。因此得出结论，体内OEP16最有可能在穿梭于种子质体外壳中的氨基酸中起作用。

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