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首页> 外文期刊>Journal of Experimental Botany >Reactive oxygen species form part of a regulatory pathway initiating trans-differentiation of epidermal transfer cells in Vicia faba cotyledons.
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Reactive oxygen species form part of a regulatory pathway initiating trans-differentiation of epidermal transfer cells in Vicia faba cotyledons.

机译：活性氧形成了蚕豆子叶表皮转移细胞启动转分化的调控途径的一部分。

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Various cell types can trans-differentiate to a transfer cell (TC) morphology characterized by deposition of polarized ingrowth walls comprised of a uniform layer on which wall ingrowths (WIs) develop. WIs form scaffolds supporting amplified plasma membrane areas enriched in transporters conferring a cellular capacity for high rates of nutrient exchange across apo- and symplasmic interfaces. The hypothesis that reactive oxygen species (ROS) are a component of the regulatory pathway inducing ingrowth wall formation was tested using Vicia faba cotyledons. Vicia faba cotyledons offer a robust experimental model to examine TC induction as, on being placed into culture, their adaxial epidermal cells rapidly (hours) form ingrowth walls on their outer periclinal walls. These are readily visualized by electron microscopy, and epidermal peels of their trans-differentiating cells allow measures of cell-specific gene expression. Ingrowth wall formation responded inversely to pharmacological manipulation of ROS levels, indicating that a flavin-containing enzyme (NADPH oxidase) and superoxide dismutase cooperatively generate a regulatory H(2)O(2) signature. Extracellular H(2)O(2) fluxes peaked prior to the appearance of WIs and were followed by a slower rise in H(2)O(2) flux that occurred concomitantly, and co-localized, with ingrowth wall formation. De-localizing the H(2)O(2) signature caused a corresponding de-localization of cell wall deposition. Temporal and epidermal cell-specific expression profiles of VfrbohA and VfrbohC coincided with those of extracellular H(2)O(2) production and were regulated by cross-talk with ethylene. It is concluded that H(2)O(2) functions, downstream of ethylene, to activate cell wall biosynthesis and direct polarized deposition of a uniform wall on which WIs form.
机译：各种细胞类型可以转分化为转移细胞（TC）形态，其特征是极化的向内生长壁的沉积，该壁由均匀的层组成，壁上向内生长（WI）。 WI形成支撑扩增的质膜区域的支架，这些区域富集了转运蛋白，赋予了细胞通过载脂蛋白和同质界面进行高速率营养交换的能力。使用蚕豆子叶（Vicia faba）子叶测试了活性氧（ROS）是诱导向内生长壁形成的调控途径的组成成分的假说。蚕豆子叶提供了一个强大的实验模型，可以检查TC的诱导，因为将其放置在培养物中后，其表皮上皮细胞迅速（数小时）在其外周壁上形成了向内生长壁。这些可以通过电子显微镜容易地看到，并且它们的转分化细胞的表皮剥离可以测量细胞特异性基因的表达。向内生长壁的形成与ROS水平的药理作用成反比，表明含黄素的酶（NADPH氧化酶）和超氧化物歧化酶协同产生调节性H（2）O（2）签名。细胞外的H（2）O（2）通量达到WIs出现之前的峰值，然后伴随着H（2）O（2）通量的缓慢上升，同时发生并共同定位，并向内生长。 H（2）O（2）签名的非本地化导致相应的细胞壁沉积的非本地化。 VfrbohA和VfrbohC的时间和表皮细胞特异性表达谱与细胞外H（2）O（2）生产的相符，并通过与乙烯的串扰来调节。结论是，H（2）O（2）在乙烯的下游起作用，以激活细胞壁生物合成并直接极化形成WI的均匀壁的沉积。

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