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首页> 外文期刊>Journal of Experimental Botany >Water permeability differs between growing and non-growing barley leaf tissues
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Water permeability differs between growing and non-growing barley leaf tissues

机译：生长和不生长的大麦叶片组织的透水性不同

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A pressure probe technique and an osmotic swelling assay were used to compare water transport properties between growing and non-growing tissues of leaf three of barley. The epidermis was analysed in planta by pressure probe, whereas (predominantly) mesophyll protoplasts were analysed by osmotic swelling. Hydraulic conductivity (Lp) and, by implication, water permeability (P(f)) of epidermal cells was 31% higher in the leaf elongation zone (Lp=0.5+/-0.2 mum s(-1) MPa(-1); P(f)=65+/-25 mum s(-1); means +/-SD of n=17 cells) than in the, non-growing, emerged leaf zone (Lp=0.4+/-0.1 mum s(-1) MPa(-1); P(f)=50+/-15 mum s(-1); n=24; P <0.05). Similarly, water permeability of mesophyll protoplasts was by 55% higher in the elongation compared with emerged leaf zone (P(f)=13+/-1 mum s(-1) compared with 8+/-1 mum s(-1); n=57 and 36 protoplasts, respectively; P <0.01). Within the leaf elongation zone, a small population of larger-sized protoplasts could be distinguished. These protoplasts, which originated most likely from parenchymateous bundle sheath or midrib parenchyma cells, had a three-fold higher water permeability (P <0.001) as mesophyll protoplasts. The effect on Lp and P(f) of known aquaporin inhibitors was tested with the pressure probe (Au(+), Ag(+), Hg(2+), phloretin) and the osmotic swelling assay (phloretin). Only phloretin, when applied to protoplasts in the swelling assay caused an average decrease in P(f), but the effect varied between isolations. Technical approaches and cell-type and growth-specific differences in water transport properties are discussed.
机译：用压力探针技术和渗透溶胀试验比较了大麦三叶生长和非生长组织之间的水传输特性。表皮通过压力探针在植物体内进行分析，而（主要）叶肉原生质体通过渗透溶胀进行分析。表皮细胞的水力传导率（Lp）和表皮细胞的水渗透性（P（f））在叶片伸长区（Lp = 0.5 +/- 0.2 ms s（-1）MPa（-1））高31％。 P（f）= 65 +/- 25 mum s（-1）;表示n = 17个细胞的+/- SD）比未生长的生叶区域（Lp = 0.4 +/- 0.1 ms s（-1）） -1）MPa（-1）; P（f）= 50 +/- 15毫米s（-1）; n = 24; P <0.05）。同样，叶肉原生质体的透水性与伸长的叶区相比延长了55％（P（f）= 13 +/- 1 mum s（-1）与8 +/- 1 ms -1（-1） ; n = 57和36原生质体; P <0.01）。在叶片伸长区内，可以分辨出一小群较大的原生质体。这些原生质体最有可能来自实质性束鞘或中肋实质细胞，其水渗透性是叶肉原生质体的三倍（P <0.001）。使用压力探针（Au（+），Ag（+），Hg（2 +），phloretin）和渗透溶胀试验（phloretin）测试了已知水通道蛋白抑制剂对Lp和P（f）的影响。在生长试验中，仅将芦荟素应用于原生质体会导致P（f）的平均下降，但在分离过程中效果会有所不同。讨论了水输送特性的技术方法，细胞类型和生长特异性差异。

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