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首页> 外文期刊>Journal of Experimental Botany >Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants
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Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants

机译：葡萄转录因子VvWRKY1的分离，鉴定及其对转基因烟草植物中真菌病原体反应的影响

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摘要

Pathogen attack represents a major problem for viticulture and for agriculture in general. At present, the use of phytochemicals is more and more restrictive, and therefore it is becoming essential to control disease by having a thorough knowledge of resistance mechanisms. The present work focused on the trans-regulatory proteins potentially involved in the control of the plant defence response, the WRKY proteins. A full-length cDNA, designated VvWRKY1, was isolated from a grape berry library (Vitis vinifera L. cv. Cabernet Sauvignon). It encodes a polypeptide of 151 amino acids whose structure is characteristic of group IIc WRKY proteins. VvWRKY1 gene expression in grape is regulated in a developmental manner in berries and leaves and by various signal molecules involved in defence such as salicylic acid, ethylene, and hydrogen peroxide. Biochemical analysis indicates that VvWRKY1 specifically interacts with the W-box in various nucleotidic contexts. Functional analysis of VvWRKY1 was performed by overexpression in tobacco, and transgenic plants exhibited reduced susceptibility to various fungi but not to viruses. These results are consistent with a possible role for VvWRKY1 in grapevine defence against fungal pathogens.
机译：病原体侵袭是葡萄栽培和整个农业的主要问题。目前，植物化学物质的使用受到越来越多的限制，因此，通过全面地了解耐药机制来控制疾病变得至关重要。目前的工作集中在可能参与植物防御反应控制的反式调节蛋白WRKY蛋白上。从葡萄浆果文库（Vitis vinifera L. cv。Cabernet Sauvignon）中分离出全长cDNA，命名为VvWRKY1。它编码具有151个氨基酸的多肽，其结构是IIc组WRKY蛋白的特征。葡萄中的VvWRKY1基因表达以发育方式在浆果和叶片中受到调节，并受参与防御的各种信号分子（如水杨酸，乙烯和过氧化氢）的调节。生化分析表明，VvWRKY1在各种核苷酸背景下与W-box特异性相互作用。 VvWRKY1的功能分析是通过在烟草中过表达进行的，转基因植物对各种真菌的敏感性降低，但对病毒的敏感性降低。这些结果与VvWRKY1在葡萄藤中抵抗真菌病原体的可能作用中相符。

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