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首页> 外文期刊>Journal of Experimental Botany >Proteomic analysis reveals differences between Vitis vinifera L. cultivars Chardonnay and Cabernet Sauvignon and their responses to water deficit and salinity.
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Proteomic analysis reveals differences between Vitis vinifera L. cultivars Chardonnay and Cabernet Sauvignon and their responses to water deficit and salinity.

机译：蛋白质组学分析揭示了葡萄品种霞多丽和赤霞珠之间的差异以及它们对水分缺乏和盐度的响应。

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摘要

The impact of water deficit and salt stress on two important wine grape cultivars, Chardonnay and Cabernet Sauvignon, was investigated. Plants were exposed to increasing salinity and water deficit stress over a 16 d time period. Measurements of stem water potentials, and shoot and leaf lengths indicated that Chardonnay was more tolerant to these stresses than Cabernet Sauvignon. Shoot tips were harvested every 8 d for proteomic analysis using a trichloroacetic acid/acetone extraction protocol and two-dimensional gel electrophoresis. Proteins were stained with Coomassie Brilliant Blue, quantified, and then 191 unique proteins were identified using matrix-assisted laser desorption ionization time of flight/time of flight mass spectrometry. Peptide sequences were matched against both the NCBI nr and TIGR Vitis expressed sequence tag (EST) databases that had been implemented with all public Vitis sequences. Approximately 44% of the protein isoforms could be identified. Analysis of variance indicated that varietal difference was the main source of protein expression variation (40%). In stressed plants, reduction of the amount of proteins involved with photosynthesis, protein synthesis, and protein destination was correlated with the inhibition of shoot elongation. Many of the proteins up-regulated in Chardonnay were of unclassified or of unknown function, whereas proteins specifically up-regulated in Cabernet Sauvignon were involved in protein metabolism..
机译：研究了水分亏缺和盐分胁迫对两个重要的酿酒葡萄品种夏敦埃酒和赤霞珠的影响。在16 d的时间内，植物暴露在盐度和水分亏缺胁迫下。茎水势，茎和叶长度的测量结果表明，霞多丽比赤霞珠对这些胁迫的耐受性更高。每三天收集一次茎尖，使用三氯乙酸/丙酮提取方案和二维凝胶电泳进行蛋白质组学分析。用考马斯亮蓝对蛋白质染色，进行定量，然后使用基质辅助激光解吸电离飞行时间/飞行时间质谱法鉴定191种独特的蛋白质。肽序列与已经使用所有公共Vitis序列实现的NCBI nr和TIGR Vitis表达序列标签（EST）数据库匹配。可以鉴定出约44％的蛋白质同工型。方差分析表明，品种差异是蛋白质表达差异的主要来源（40％）。在胁迫的植物中，参与光合作用，蛋白质合成和蛋白质转运的蛋白质数量的减少与芽伸长的抑制相关。在霞多丽中上调的许多蛋白质未分类或功能未知，而在赤霞珠中特别上调的蛋白质则参与蛋白质代谢。

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