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首页> 外文期刊>Journal of Experimental Botany >Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion.
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Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion.

机译：硝酸盐消耗条件下蓝藻糖原生物合成的动态代谢图谱。

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摘要

Cyanobacteria represent a globally important biomass because they are responsible for a substantial proportion of primary production in the hydrosphere. Arthrospira platensis is a fast-growing halophilic cyanobacterium capable of accumulating glycogen and has the potential to serve as a feedstock in the fermentative production of third-generation biofuels. Accordingly, enhancing cyanobacterial glycogen production is a promising biofuel production strategy. However, the regulatory mechanism of glycogen metabolism in cyanobacteria is poorly understood. The aim of the present study was to determine the metabolic flux of glycogen biosynthesis using a dynamic metabolomic approach. Time-course profiling of widely targeted cyanobacterial metabolic intermediates demonstrated a global metabolic reprogramming that involves transient increases in the levels of some amino acids during the glycogen production phase induced by nitrate depletion. Also, in vivo labelling with NaH13CO3 enabled direct measurement of metabolic intermediate turnover in A. platensis, revealing that under conditions of nitrate depletion glycogen is biosynthesized with carbon derived from amino acids released from proteins via gluconeogenesis. This dynamic metabolic profiling approach provided conclusive evidence of temporal alterations in the metabolic profile in cyanobacterial cells.
机译：蓝细菌代表了全球重要的生物质，因为它们对水圈初级生产的很大一部分负责。 Arthrospira platensis是一种快速生长的嗜盐蓝细菌，能够积累糖原，并具有作为第三代生物燃料发酵生产中的原料的潜力。因此，增强蓝细菌糖原的生产是一种有前途的生物燃料生产策略。然而，对蓝细菌糖原代谢的调控机制了解甚少。本研究的目的是使用动态代谢组学方法确定糖原生物合成的代谢通量。广泛针对性的蓝细菌代谢中间体的时程分析表明，全局代谢重编程涉及在硝酸盐消耗诱导的糖原产生阶段某些氨基酸水平的瞬时增加。此外，在体内用NaH 13 CO 3 进行标记可以直接测量白僵菌的代谢中间更新，这表明在硝酸盐消耗的条件下，糖原是由以下来源的碳生物合成的通过糖异生从蛋白质释放的氨基酸。这种动态代谢谱分析方法为蓝细菌细胞中代谢谱的时间变化提供了确凿的证据。

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