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首页> 外文期刊>Journal of Experimental Botany >Influence of carbon to nitrogen ratios on soybean somatic embryo (cv. Jack) growth and composition.
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Influence of carbon to nitrogen ratios on soybean somatic embryo (cv. Jack) growth and composition.

机译：碳氮比对大豆体细胞胚（杰克）生长和组成的影响。

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摘要

Soybean [Glycine max (L.) Merr.] seed are valued for their protein and oil content. Soybean somatic embryos cultured in Soybean Histodifferentiation and Maturation (SHaM) medium were examined for their suitability as a model system for developing an understanding of assimilate partitioning and metabolic control points for protein and oil biosynthesis in soybean seed. This report describes the growth dynamics and compositional changes of SHaM embryos in response to change in the carbon to nitrogen ratio of the medium. It was postulated that at media compositions that were sufficient to support maximal growth rates, changes in the C:N ratio are likely to influence the partitioning of resources between the various storage products, especially protein and oil. As postulated, at steady-state growth rates, embryo protein content was strongly correlated with decreasing C:N ratios and increasing glutamine consumption rates. However, oil content remained relatively unchanged across the C:N ratio range tested, and resources were instead directed towards the starch and residual biomass (estimated by mass balance) pools in response to increasing C:N ratios. Protein and oil were inversely related only at concentrations of sucrose in the medium <88 mM, where carbon limited growth and no starch was found to accumulate in the tissues. These observations and the high reproducibility in the data indicate that SHaM embryos are an ideal model system for the application of metabolic flux analysis studies designed to test hypotheses regarding assimilate partitioning in developing soybean seeds.
机译：大豆[Glycine max（L.）Merr。]种子的蛋白质和油含量很高。研究了在大豆组织分化和成熟（SHaM）培养基中培养的大豆体细胞胚的适宜性，以此作为模型系统，可用于理解大豆种子中蛋白质和油脂生物合成的同化分配和代谢控制点。该报告描述了SHaM胚胎的生长动力学和组成变化，以响应培养基中碳氮比的变化。据推测，在足以支持最大生长速率的培养基组成下，C：N比的变化可能会影响各种存储产品（尤其是蛋白质和油脂）之间的资源分配。如所假定的，在稳态生长速率下，胚蛋白含量与降低的C：N比和增加的谷氨酰胺消耗率密切相关。但是，在测试的C：N比率范围内，油含量保持相对不变，并且响应于C：N比率的增加，资源转向淀粉和残留生物量（通过质量平衡估算）。蛋白质和油仅在<88 mM的蔗糖浓度下呈负相关，在碳浓度下，碳的生长受到限制，并且组织中没有淀粉积聚。这些观察结果和数据的高度可重复性表明，SHaM胚胎是代谢通量分析研究应用的理想模型系统，旨在研究关于发育中的大豆种子中同化物分配的假设。

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