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首页> 外文期刊>Journal of Experimental Botany >TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins.
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TGA transcription factors and jasmonate-independent COI1 signalling regulate specific plant responses to reactive oxylipins.

机译：TGA转录因子和不依赖茉莉酸酯的COI1信号调节植物对反应性脂蛋白的特异性反应。

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摘要

Jasmonates and phytoprostanes are oxylipins that regulate stress responses and diverse physiological and developmental processes. 12-Oxo-phytodienoic acid (OPDA) and phytoprostanes are structurally related electrophilic cyclopentenones, which activate similar gene expression profiles that are for the most part different from the action of the cyclopentanone jasmonic acid (JA) and its biologically active amino acid conjugates. Whereas JA-isoleucine signals through binding to COI1, the bZIP transcription factors TGA2, TGA5, and TGA6 are involved in regulation of gene expression in response to phytoprostanes. Here root growth inhibition and target gene expression were compared after treatment with JA, OPDA, or phytoprostanes in mutants of the COI1/MYC2 pathway and in different TGA factor mutants. Inhibition of root growth by phytoprostanes was dependent on COI1 but independent of jasmonate biosynthesis. In contrast, phytoprostane-responsive gene expression was strongly dependent on TGA2, TGA5, and TGA6, but not dependent on COI1, MYC2, TGA1, and TGA4. Different mutant and overexpressing lines were used to determine individual contributions of TGA factors to cyclopentenone-responsive gene expression. Whereas OPDA-induced expression of the cytochrome P450 gene CYP81D11 was primarily regulated by TGA2 and TGA5, the glutathione S-transferase gene GST25 and the OPDA reductase gene OPR1 were regulated by TGA5 and TGA6, but less so by TGA2. These results support the model that phytoprostanes and OPDA regulate differently (i) growth responses, which are COI1 dependent but jasmonate independent; and (ii) lipid stress responses, which are strongly dependent on TGA2, TGA5, and TGA6. Identification of molecular components in cyclopentenone signalling provides an insight into novel oxylipin signal transduction pathways.
机译：茉莉酸酯和植物前列腺素是调节压力反应以及各种生理和发育过程的脂蛋白。 12-氧-植物二烯酸（OPDA）和植物前列腺素是结构相关的亲电环戊烯酮，它们激活相似的基因表达谱，这些谱图谱与环戊酮茉莉酸（JA）及其生物活性氨基酸缀合物的作用在很大程度上不同。 JA-异亮氨酸通过结合COI1发出信号，而bZIP转录因子TGA2，TGA5和TGA6参与了对植物前列腺素的基因表达调控。在COI1 / MYC2途径的突变体和不同的TGA因子突变体中，用JA，ODPA或植物前列腺素处理后，比较了根生长抑制和靶基因表达。植物前列腺素对根系生长的抑制取决于COI1，但与茉莉酸酯的生物合成无关。相反，植物前列腺素反应性基因表达强烈依赖于TGA2，TGA5和TGA6，而不依赖于COI1，MYC2，TGA1和TGA4。使用不同的突变体和过表达系来确定TGA因子对环戊烯酮反应性基因表达的个体贡献。 OPDA诱导的细胞色素P450基因CYP81D11的表达主要受TGA2和TGA5调控，而谷胱甘肽S转移酶基因GST25和OPDA还原酶基因OPR1受TGA5和TGA6调控，而TGA2则较少。这些结果支持了植物前列腺素和OPDA调节不同的模型（i）生长反应，其依赖于COI1但不依赖于茉莉酮酸酯; （ii）脂质应激反应，其强烈依赖于TGA2，TGA5和TGA6。环戊烯酮信号传导中分子成分的鉴定提供了对新型羟脂信号转导途径的了解。

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