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首页> 外文期刊>Journal of Experimental Botany >Induction of cotton ovule culture fibre branching by co-expression of cotton BTL, cotton SIM, and Arabidopsis STI genes.
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Induction of cotton ovule culture fibre branching by co-expression of cotton BTL, cotton SIM, and Arabidopsis STI genes.

机译：棉花BTL，棉花SIM和拟南芥STI基因的共表达诱导棉花胚珠培养纤维分支。

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摘要

The highly elongated single-celled cotton fibre consists of lint and fuzz, similar to the Arabidopsis trichome. Endoreduplication is an important determinant in Arabidopsis trichome initiation and morphogenesis. Fibre development is also controlled by functional homologues of Arabidopsis trichome patterning genes, although fibre cells do not have a branched shape like trichomes. The identification and characterization of the homologues of 10 key Arabidopsis trichome branching genes in Gossypium arboreum are reported here. Nuclear ploidy of fibres was determined, and gene function in cotton callus and fibre cells was investigated. The results revealed that the nuclear DNA content was constant in fuzz, whereas a limited and reversible change occurred in lint after initiation. Gossypeum arboreum BRANCHLESS TRICHOMES (GaBLT) was not transcribed in fibres. The homologue of STICHEL (STI), which is essential for trichome branching, was a pseudogene in Gossypium. Targeted expression of GaBLT, Arabidopsis STI, and the cytokinesis-repressing GaSIAMESE in G. hirsutum fibre cells cultured in vitro resulted in branching. The findings suggest that the distinctive developmental mechanism of cotton fibres does not depend on endoreduplication. This important component may be a relic function that can be activated in fibre cells.
机译：高度伸长的单细胞棉纤维由绒毛和绒毛组成，类似于拟南芥的三角毛。核内复制是拟南芥毛状体起始和形态发生的重要决定因素。纤维发育也受拟南芥毛状体模式基因的功能同源物控制，尽管纤维细胞不像毛状体那样具有分支形状。本文报道了棉铃兰中10个关键拟南芥毛状体分支基因的同源物的鉴定和表征。确定了纤维的核倍性，并研究了棉花愈伤组织和纤维细胞中的基因功能。结果表明，绒毛中的核DNA含量是恒定的，而棉绒在启动后发生了有限且可逆的变化。棉纤维无分支毛囊（GaBLT）未转录到纤维中。对毛状体分支至关重要的STICHEL（STI）的同源物是棉的假基因。 GaBLT，拟南芥STI和抑制胞质分裂的GaSIAMESE在体外培养的hirstum纤维细胞中的靶向表达导致分支。这些发现表明，棉纤维独特的发育机制不依赖于核内复制。此重要组件可能是可以在纤维细胞中激活的遗迹功能。

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