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首页> 外文期刊>Journal of Experimental Botany >The cytoskeleton is disrupted by the bacterial effector HrpZ, but not by the bacterial PAMP flg22, in tobacco BY-2 cells.
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The cytoskeleton is disrupted by the bacterial effector HrpZ, but not by the bacterial PAMP flg22, in tobacco BY-2 cells.

机译：在烟草BY-2细胞中，细胞骨架被细菌效应子HrpZ破坏，但未被细菌PAMP flg22破坏。

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摘要

Plant innate immunity is composed of two layers. Basal immunity is triggered by pathogen-associated molecular patterns (PAMPs) such as the flagellin-peptide flg22 and is termed PAMP-triggered immunity (PTI). In addition, effector-triggered immunity (ETI) linked with programmed cell death and cytoskeletal reorganization can be induced by pathogen-derived factors, such as the Harpin proteins originating from phytopathogenic bacteria. To get insight into the link between cytoskeleton and PTI or ETI, this study followed the responses of actin filaments and microtubules to flg22 and HrpZ in vivo by spinning-disc confocal microscopy in GFP-tagged marker lines of tobacco BY-2. At a concentration that clearly impairs mitosis, flg22 can induce only subtle cytoskeletal responses. In contrast, HrpZ causes a rapid and massive bundling of actin microfilaments (completed in ~20 min, i.e. almost simultaneously with extracellular alkalinization), which is followed by progressive disintegration of actin cables and cytoplasmic microtubules, a loss of cytoplasmic structure, and vacuolar disintegration. Cytoskeletal disruption is proposed as an early event that discriminates HrpZ-triggered ETI-like defence from flg22-triggered PTI.
机译：植物先天免疫由两层组成。基础免疫是由病原体相关分子模式（PAMP）（例如鞭毛蛋白肽flg22）触发的，被称为PAMP触发免疫（PTI）。另外，与病原体衍生的因子（例如源自植物病原性细菌的Harpin蛋白）可以诱导与程序性细胞死亡和细胞骨架重组相关的效应触发免疫（ETI）。为了深入了解细胞骨架与PTI或ETI之间的联系，本研究通过旋转盘共聚焦显微镜在烟草BY-2标记的GFP标记系中追踪了肌动蛋白丝和微管在体内对flg22和HrpZ的反应。在明显损害有丝分裂的浓度下，flg22只能诱导细微的细胞骨架反应。相比之下，HrpZ导致肌动蛋白微丝快速大量束缚（在约20分钟内完成，即几乎与细胞外碱化同时发生），随后肌动蛋白电缆和胞质微管逐渐崩解，胞质结构丧失和液泡崩解。提议将细胞骨架破坏作为一种早期事件来区分HrpZ触发的ETI样防御与flg22触发的PTI。

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