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首页> 外文期刊>Journal of Experimental Botany >Proline-rich protein-like PRPL1 controls elongation of root hairs in Arabidopsis thaliana
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Proline-rich protein-like PRPL1 controls elongation of root hairs in Arabidopsis thaliana

机译：富含脯氨酸的蛋白样PRPL1控制拟南芥根毛的伸长

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摘要

The synthesis and composition of cell walls is dynamically adapted in response to many developmental and environmental signals. In this respect, cell wall proteins involved in controlling cell elongation are critical for cell development. Transcriptome analysis identified a gene in Arabidopsis thaliana, which was named proline-rich protein-like, AtPRPL1, based on sequence similarities from a phylogenetic analysis. The most resemblance was found to AtPRP1 and AtPRP3 from Arabidopsis, which are known to be involved in root hair growth and development. In A. thaliana four proline-rich cell wall protein genes, playing a role in building up the cross-connections between cell wall components, can be distinguished. AtPRPL1 is a small gene that in promoter:: GUS (beta-glucuronidase) analysis has high expression in trichoblast cells and in the collet. Chemical or mutational interference with root hair formation inhibited this expression. Altered expression levels in knock-out or overexpression lines interfered with normal root hair growth and etiolated hypocotyl development, but Fourier transform-infrared (FT-IR) analysis did not identify consistent changes in cell wall composition of root hairs and hypocotyl. Co-localization analysis of the AtPRPL1-green fluorescent protein (GFP) fusion protein and different red fluorescent protein (RFP)-labelled markers confirmed the presence of AtPRPL1-GFP in small vesicles moving over the endoplasmic reticulum. Together, these data indicate that the AtPRPL1 protein is involved in the cell's elongation process. How exactly this is achieved remains unclear at present.
机译：细胞壁的合成和组成可以响应许多发育和环境信号而动态适应。在这方面，参与控制细胞伸长的细胞壁蛋白对于细胞发育至关重要。转录组分析基于系统进化分析中的序列相似性，在拟南芥中鉴定了一个基因，该基因被命名为富含脯氨酸的蛋白样AtPRPL1。发现与拟南芥的AtPRP1和AtPRP3最相似，已知它们与根毛的生长和发育有关。在拟南芥中，可以区分出四个富含脯氨酸的细胞壁蛋白基因，它们在建立细胞壁成分之间的交叉连接中起着作用。 AtPRPL1是一个小基因，在启动子:: GUS（β-葡萄糖醛酸苷酶）分析中，在单核细胞和筒夹中高表达。对根毛形成的化学或突变干扰抑制了该表达。基因敲除或过表达系中表达水平的改变会干扰正常的根毛生长和黄化的下胚轴发育，但傅立叶变换红外（FT-IR）分析未发现根毛和下胚轴的细胞壁组成发生一致变化。 AtPRPL1绿色荧光蛋白（GFP）融合蛋白和不同的红色荧光蛋白（RFP）标记的标记物的共定位分析证实，AtPRPL1-GFP存在于内质网上方的小囊泡中。总之，这些数据表明AtPRPL1蛋白与细胞的延伸过程有关。目前尚不清楚如何实现这一目标。

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