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首页> 外文期刊>Journal of Experimental Botany >The BEL1-like family of transcription factors in potato
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The BEL1-like family of transcription factors in potato

机译：马铃薯中的BEL1样转录因子家族

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BEL1-type proteins are ubiquitous plant transcription factors in the three-amino-acid-loop-extension superfamily. They interact with KNOTTED1-like proteins, and function as heterodimers in both floral and vegetative development. Using the yeast two-hybrid system with POTATO HOMEOBOX1 (POTH1) as the bait, seven BEL1-type proteins were originally identified. One of these genes, designated StBEL5, has transcripts that move long distances in the plant and enhance tuberization and root growth. Using the potato genome database, 13 active BEL1-like genes were identified that contain the conserved homeobox domain and the BELL domain, both of which are essential for the function of BEL1-type proteins. Phylogenetic analysis of the StBEL family demonstrated a degree of orthology with the 13 BEL1-like genes of Arabidopsis. A profile of the gene structure of the family revealed conservation of the length and splicing patterns of internal exons that encode key functional domains. Yeast two-hybrid experiments with KNOTTED1-like proteins and the new StBELs confirmed the interactive network between these two families. Analyses of RNA abundance patterns clearly showed that three StBEL genes, BEL5, -11, and -29, make up approximately two-thirds of the total transcript values for the entire family. Among the 10 organs evaluated here, these three genes exhibited the 12 greatest transcript abundance values. Using a phloem-transport induction system and gel-shift assays, transcriptional cross-regulation within the StBEL family was confirmed. Making use of the potato genome and current experimental data, a comprehensive profile of the StBEL family is presented in this study.
机译：BEL1型蛋白是三氨基酸环延伸超家族中普遍存在的植物转录因子。它们与类似KNOTTED1的蛋白质相互作用，并在花卉和营养发育中充当异二聚体。使用以POTATO HOMEOBOX1（POTH1）为诱饵的酵母双杂交系统，最初鉴定出7种BEL1型蛋白。这些基因之一称为StBEL5，其转录本可以在植物中长距离移动并增强块茎和根的生长。使用马铃薯基因组数据库，鉴定了13个活跃的BEL1类基因，它们包含保守的同源盒结构域和BELL结构域，这两者对于BEL1型蛋白的功能都是必不可少的。对StBEL家族的系统发育分析表明，拟南芥的13个BEL1样基因具有一定的正交性。该家族基因结构的概况揭示了编码关键功能域的内部外显子的长度和剪接模式的保守性。酵母样KNOTTED1蛋白和新的StBELs的两个杂交实验证实了这两个家族之间的相互作用网络。 RNA丰度模式的分析清楚地表明，三个StBEL基因BEL5，-11和-29约占整个家族总转录本值的三分之二。在这里评估的10个器官中，这3个基因表现出12个最大的转录物丰度值。使用韧皮部转运诱导系统和凝胶移位测定法，证实了StBEL家族内的转录交叉调节。利用马铃薯基因组和当前的实验数据，在这项研究中提供了StBEL家族的全面概况。

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