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首页> 外文期刊>Journal of Experimental Botany >Nitrosative stress triggers microtubule reorganization in Arabidopsis thaliana
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Nitrosative stress triggers microtubule reorganization in Arabidopsis thaliana

机译：亚硝化胁迫触发拟南芥中的微管重组。

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摘要

Microtubules (MTs) are essential components of the cytoskeleton and fulfil multiple cellular functions in developmental processes, readily responding to intrinsic and external cues. Nitric oxide signalling is well established in plants, and the MT cytoskeleton is one of its potential targets. To mimic low level nitrosative stress, growth medium was supplemented with 3-nitro-l-tyrosine (NO2-Tyr), a nitrated form of the amino acid tyrosine, and concentration-dependent changes in root growth rate and a reduction in cell division frequencies in Arabidopsis thaliana were observed. In addition, it is reported that exposure to low NO2-Tyr concentrations was not detrimental to plant health and caused subtle and reversible defects. In contrast, growth defects caused by high NO2-Tyr concentrations could not be reversed. Live cell imaging of an MT reporter line revealed that treatment with a low concentration of NO2-Tyr correlated with disorganized cortical MT arrays and associated non-polar cell expansion in the elongation zone. NO(2)Tyr treatment antagonized the effects of taxol and oryzalin, further supporting the association of NO2-Tyr with MTs. Furthermore, oblique division plane orientations were observed which were probably induced prior to cytokinesis.
机译：微管（MTs）是细胞骨架的重要组成部分，在发育过程中具有多种细胞功能，可以轻松响应内在和外在线索。一氧化氮信号已经在植物中得到了很好的确立，MT细胞骨架是其潜在的靶标之一。为了模拟低水平的亚硝化胁迫，在生长培养基中添加了3-硝基-1-酪氨酸（NO2-Tyr），氨基酸酪氨酸的硝化形式以及根部生长速率的浓度依赖性变化和细胞分裂频率的降低在拟南芥中观察到。另外，据报道暴露于低的NO 2 -Tyr浓度对植物健康无害并引起细微和可逆的缺陷。相反，由高NO2-Tyr浓度引起的生长缺陷无法逆转。 MT报告基因系的活细胞成像显示，低浓度的NO2-Tyr处理与皮层MT阵列紊乱以及伸长区域中非极性细胞扩增相关。 NO（2）Tyr治疗可拮抗紫杉醇和谷氨酰胺的作用，进一步支持NO2-Tyr与MT的缔合。此外，观察到倾斜的分裂平面取向，其可能在胞质分裂之前被诱导。

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