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首页> 外文期刊>Journal of Experimental Botany >Transcriptional responses indicate maintenance of photosynthetic proteins as key to the exceptional chilling tolerance of C-4 photosynthesis in Miscanthus x giganteus
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Transcriptional responses indicate maintenance of photosynthetic proteins as key to the exceptional chilling tolerance of C-4 photosynthesis in Miscanthus x giganteus

机译：转录反应表明光合作用蛋白的维持是Miscanthus x giganteus对C-4光合作用超强耐寒性的关键

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摘要

Miscanthus x giganteus is exceptional among C-4 plants in its ability to acclimate to chilling (<= 14 degrees C) and maintain a high photosynthetic capacity, in sharp contrast to maize, leading to very high productivity even in cool temperate climates. To identify the mechanisms that underlie this acclimation, RNA was isolated from M x giganteus leaves in chilling and nonchilling conditions and hybridized to microarrays developed for its close relative Zea mays. Among 21 000 array probes that yielded robust signals, 723 showed significant expression change under chilling. Approximately half of these were for annotated genes. Thirty genes associated with chloroplast membrane function were all upregulated. Increases in transcripts for the lhcb5 (chlorophyll a/b-binding protein CP26), ndhF (NADH dehydrogenase F, chloroplast), atpA (ATP synthase alpha subunit), psbA (D1), petA (cytochrome f), and lhcb4 (chlorophyll a/b-binding protein CP29), relative to housekeeping genes in M. x giganteus, were confirmed by quantitative reverse-transcription PCR. In contrast, psbo1, lhcb5, psbA, and lhcb4 were all significantly decreased in Z. mays after 14 days of chilling. Western blot analysis of the D1 protein and LHCII type II chlorophyll a/b-binding protein also showed significant increases in M. x giganteus during chilling and significant decreases in Z. mays. Compared to other C4 species, M. x giganteus grown in chilling conditions appears to counteract the loss of photosynthetic proteins and proteins protecting photosystem II typically observed in other species by increasing mRNA levels for their synthesis.
机译：与C-4植物相比，Miscanthus x giganteus具有出色的适应气候变化的能力（<= 14摄氏度）并保持较高的光合作用能力，与玉米形成鲜明对比，即使在凉爽的温带气候下也能保持很高的生产力。为了确定这种适应的机制，在低温和非低温条件下从Mx giganteus叶片中分离RNA，并与为其近缘玉米而开发的微阵列杂交。在21000个产生稳健信号的阵列探针中，有723个在冷冻条件下显示出明显的表达变化。其中大约一半用于注释的基因。与叶绿体膜功能相关的三十个基因均被上调。 lhcb5（叶绿素a / b结合蛋白CP26），ndhF（NADH脱氢酶F，叶绿体），atpA（ATP合酶α亚基），psbA（D1），petA（细胞色素f）和lhcb4（叶绿素a）的转录本增加通过定量逆转录PCR证实了相对于硕大冈比亚牛的管家基因而言的β-b结合蛋白CP29）。相反，冷藏14天后，玉米中的psbo1，lhcb5，psbA和lhcb4均显着降低。 D1蛋白和LHCII II型叶绿素a / b结合蛋白的蛋白质印迹分析还显示，在冷却过程中，巨型M. x giganteus明显增加，而Z. mays则明显减少。与其他C4物种相比，在寒冷条件下生长的M. x giganteus似乎可以通过增加其合成的mRNA水平来抵消其他物种通常观察到的光合作用蛋白和保护光系统II的蛋白的损失。

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