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首页> 外文期刊>Journal of Experimental Botany >Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana
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Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana

机译：黄瓜金属转运蛋白MTP8在酵母和拟南芥中表达时，对锰的耐受性增强

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摘要

Cation diffusion facilitator (CDF) proteins are ubiquitous divalent cation transporters that have been proved to be essential for metal homeostasis and tolerance in Archaebacteria, Bacteria, and Eukaryota. In plants, CDFs are designated as metal tolerance proteins (MTPs). Due to the lack of genomic resources, studies on MTPs in other plants, including cultivated crops, are lacking. Here, the identification and organization of genes encoding members of the MTP family in cucumber are described. The first functional characterization of a cucumber gene encoding a member of the Mn-CDF subgroup of CDF proteins, designated as CsMTP8 based on the highest homology to plant MTP8, is also presented. The expression of CsMTP8 in Saccharomyces cerevisiae led to increased Mn accumulation in yeast cells and fully restored the growth of mutants hypersensitive to Mn in Mn excess. Similarly, the overexpression of CsMTP8 in Arabidopsis thaliana enhanced plant tolerance to high Mn in nutrition media as well as the accumulation of Mn in plant tissues. When fused to green fluorescent protein (GFP), CsMTP8 localized to the vacuolar membranes in yeast cells and to Arabidopsis protoplasts. In cucumber, CsMTP8 was expressed almost exclusively in roots, and the level of gene transcript was markedly up-regulated or reduced under elevated Mn or Mn deficiency, respectively. Taken together, the results suggest that CsMTP8 is an Mn transporter localized in the vacuolar membrane, which participates in the maintenance of Mn homeostasis in cucumber root cells.
机译：阳离子扩散促进剂（CDF）蛋白是普遍存在的二价阳离子转运蛋白，已被证明对于古细菌，细菌和真核生物的金属稳态和耐受性至关重要。在植物中，CDF被指定为金属耐受蛋白（MTP）。由于缺乏基因组资源，因此缺乏对包括种植作物在内的其他植物中MTP的研究。在这里，描述了编码和编码黄瓜中MTP家族成员的基因的组织。还提出了基于与植物MTP8的最高同源性而编码为CDF蛋白的Mn-CDF亚组成员的黄瓜基因的第一个功能表征，命名为CsMTP8。 CsMTP8在酿酒酵母中的表达导致Mn在酵母细胞中的积累增加，并在Mn过量时完全恢复了对Mn过敏的突变体的生长。同样，拟南芥中CsMTP8的过表达增强了植物对营养培养基中高锰的耐受性以及植物组织中锰的积累。当与绿色荧光蛋白（GFP）融合时，CsMTP8定位在酵母细胞的液泡膜和拟南芥的原生质体中。在黄瓜中，CsMTP8几乎只在根中表达，而在Mn升高或Mn缺乏时，基因转录水平显着上调或降低。两者合计，结果表明CsMTP8是位于液泡膜中的Mn转运蛋白，其参与黄瓜根细胞中Mn稳态的维持。

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